Počet záznamů: 1
A quantitative description of light-limited cyanobacterial growth using flux balance analysis
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SYSNO ASEP 0599393 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A quantitative description of light-limited cyanobacterial growth using flux balance analysis Tvůrce(i) Höper, D. (DE)
Komkova, D. (DE)
Zavřel, Tomáš (UEK-B) RID, SAI, ORCID
Steuer, R. (DE)Celkový počet autorů 4 Číslo článku e1012280 Zdroj.dok. PLoS Computational Biology - ISSN 1553-734X
Roč. 20, č. 8 (2024)Poč.str. 27 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Computational Biology ; Cyanobacteria ; Metabolic Flux Analysis ; photosynthesis ; light Vědní obor RIV IN - Informatika Obor OECD Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8) Způsob publikování Open access Institucionální podpora UEK-B - RVO:86652079 UT WOS 001422919200008 EID SCOPUS 85200576529 DOI https://doi.org/10.1371/journal.pcbi.1012280 Anotace The metabolism of phototrophic cyanobacteria is an integral part of global biogeochemical cycles, and the capability of cyanobacteria to assimilate atmospheric CO2 into organic carbon has manifold potential applications for a sustainable biotechnology. To elucidate the properties of cyanobacterial metabolism and growth, computational reconstructions of genome-scale metabolic networks play an increasingly important role. Here, we present an updated reconstruction of the metabolic network of the cyanobacterium Synechocystis sp. PCC 6803 and its quantitative evaluation using flux balance analysis (FBA). To overcome limitations of conventional FBA, and to allow for the integration of experimental analyses, we develop a novel approach to describe light absorption and light utilization within the framework of FBA. Our approach incorporates photoinhibition and a variable quantum yield into the constraint-based description of light-limited phototrophic growth. We show that the resulting model is capable of predicting quantitative properties of cyanobacterial growth, including photosynthetic oxygen evolution and the ATP/NADPH ratio required for growth and cellular maintenance. Our approach retains the computational and conceptual simplicity of FBA and is readily applicable to other phototrophic microorganisms. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2025 Elektronická adresa https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1012280
Počet záznamů: 1