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SOS1 tonoplast neo-localization and the RGG protein SALTY are important in the extreme salinity tolerance of Salicornia bigelovii
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SYSNO ASEP 0599179 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název SOS1 tonoplast neo-localization and the RGG protein SALTY are important in the extreme salinity tolerance of Salicornia bigelovii Tvůrce(i) Salazar, O. R. (SA)
Chen, K. (CN)
Melino, V. J. (SA)
Reddy, M. P. (SA)
Hřibová, Eva (UEB-Q) RID, ORCID
Čížková, Jana (UEB-Q) RID, ORCID
Beránková, Denisa (UEB-Q) ORCID
Vega, J. P. A. (SA)
Leal, L. M. C. (SA)
Aranda, M. (SA)
Jaremko, Ł. (SA)
Jaremko, M. (SA)
Fedoroff, N. V. (US)
Tester, M. (SA)
Schmoeckel, S. M. (DE)Celkový počet autorů 15 Číslo článku 4279 Zdroj.dok. Nature Communications. - : Nature Publishing Group - ISSN 2041-1723
Roč. 15, č. 1 (2024)Poč.str. 21 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova multiple sequence alignment ; na+/h+ exchange activity ; plasma-membrane ; arabidopsis-thaliana ; proteomics data ; endoplasmic-reticulum ; increased vacuolar ; phase-separation ; genome sequence ; gene ontology Obor OECD Cell biology Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 001228176400004 EID SCOPUS 85193752724 DOI https://doi.org/10.1038/s41467-024-48595-5 Anotace The identification of genes involved in salinity tolerance has primarily focused on model plants and crops. However, plants naturally adapted to highly saline environments offer valuable insights into tolerance to extreme salinity. Salicornia plants grow in coastal salt marshes, stimulated by NaCl. To understand this tolerance, we generated genome sequences of two Salicornia species and analyzed the transcriptomic and proteomic responses of Salicornia bigelovii to NaCl. Subcellular membrane proteomes reveal that SbiSOS1, a homolog of the well-known SALT-OVERLY-SENSITIVE 1 (SOS1) protein, appears to localize to the tonoplast, consistent with subcellular localization assays in tobacco. This neo-localized protein can pump Na+ into the vacuole, preventing toxicity in the cytosol. We further identify 11 proteins of interest, of which SbiSALTY, substantially improves yeast growth on saline media. Structural characterization using NMR identified it as an intrinsically disordered protein, localizing to the endoplasmic reticulum in planta, where it can interact with ribosomes and RNA, stabilizing or protecting them during salt stress. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2025 Elektronická adresa https://doi.org/10.1038/s41467-024-48595-5
Počet záznamů: 1