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A thylakoid biogenesis BtpA protein is required for the initial step of tetrapyrrole biosynthesis in cyanobacteria
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SYSNO ASEP 0598265 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A thylakoid biogenesis BtpA protein is required for the initial step of tetrapyrrole biosynthesis in cyanobacteria Tvůrce(i) Skotnicová, Petra (MBU-M) ORCID, RID
Srivastava, Amit (MBU-M) ORCID, RID
Aggarwal, Divya (MBU-M)
Talbot, Jana (MBU-M) ORCID, RID
Karlínová, I. (CZ)
Moos, M. (CZ)
Mareš, Jan (MBU-M) ORCID, RID
Bučinská, Lenka (MBU-M) RID
Koník, Peter (MBU-M) ORCID, RID
Šimek, P. (CZ)
Tichý, Martin (MBU-M) RID, ORCID
Sobotka, Roman (MBU-M) RID, ORCIDZdroj.dok. New Phytologist - ISSN 0028-646X
Roč. 241, č. 3 (2024), s. 1236-1249Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova synechocystis sp pcc-6803 ; sp pcc 6803 ; photosystem-ii ; heat-repeats ; chlorophyll ; identification ; membrane ; isca ; accumulation ; stabilizes ; BtpA ; cyanobacteria ; glutamyl-tRNA reductase ; Synechocystis sp. PCC 6803 ; tetrapyrrole biosynthesis Obor OECD Microbiology CEP GX19-29225X GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 001107509000001 EID SCOPUS 85177479911 DOI https://doi.org/10.1111/nph.19397 Anotace Biogenesis of the photosynthetic apparatus requires complicated molecular machinery, individual components of which are either poorly characterized or unknown. The BtpA protein has been described as a factor required for the stability of photosystem I (PSI) in cyanobacteria, however, how the BtpA stabilized PSI remains unexplained.To clarify the role of BtpA, we constructed and characterized the btpA-null mutant (Delta btpA) in the cyanobacterium Synechocystis sp. PCC 6803. The mutant contained only c. 1% of chlorophyll and nearly no thylakoid membranes. However, this strain, growing only in the presence of glucose, was genetically unstable and readily generated suppressor mutations that restore the photoautotrophy.Two suppressor mutations were mapped into the hemA gene encoding glutamyl-tRNA reductase (GluTR) the first enzyme of tetrapyrrole biosynthesis. Indeed, the GluTR was not detectable in the Delta btpA mutant and the suppressor mutations restored biosynthesis of tetrapyrroles and photoautotrophy by increased GluTR expression or by improved GluTR stability/processivity. We further demonstrated that GluTR associates with a large BtpA oligomer and that BtpA is required for the stability of GluTR.Our results show that the BtpA protein is involved in the biogenesis of photosystems at the level of regulation of tetrapyrrole biosynthesis. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2025 Elektronická adresa https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.19397
Počet záznamů: 1