Počet záznamů: 1
Depletion of calpain2 accelerates epithelial barrier establishment and reduces growth factor-induced cell scattering
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SYSNO ASEP 0588259 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Depletion of calpain2 accelerates epithelial barrier establishment and reduces growth factor-induced cell scattering Tvůrce(i) Rasl, Jan (MBU-M) ORCID
Čáslavský, Josef (MBU-M)
Grušanovič, Josipa (MBU-M)
Chvalová, Věra (MBU-M) ORCID
Kosla, Jan (UMG-J) RID
Adamec, J. (US)
Groušl, Tomáš (MBU-M) RID, ORCID
Klímová, Zuzana (MBU-M) RID, ORCID
Vomastek, Tomáš (MBU-M) RID, ORCIDCelkový počet autorů 9 Číslo článku 111295 Zdroj.dok. Cellular Signalling. - : Elsevier - ISSN 0898-6568
Roč. 121, September 2024 (2024)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova mediated proteolysis ; focal adhesion ; integrin engagement ; adherens junctions ; crystal-structure ; plasma-membrane ; beta-catenin ; mu-calpain ; e-cadherin ; kinase ; Calpains ; Proteases ; Epithelial polarity ; Transepithelial electrical resistance ; Tight junctions ; Actin ; Adherens junctions ; Focal adhesions ; erk ; Migration ; Cell scattering ; hgf/sf Obor OECD Cell biology CEP GA19-08013S GA ČR - Grantová agentura ČR NU23-03-00557 GA MZd - Ministerstvo zdravotnictví LM2023050 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 ; UMG-J - RVO:68378050 UT WOS 001271754400001 EID SCOPUS 85198270418 DOI https://doi.org/10.1016/j.cellsig.2024.111295 Anotace Calpain2 is a conventional member of the non-lysosomal calpain protease family that has been shown to affect the dynamics of focal and cell-cell adhesions by proteolyzing the components of adhesion complexes. Here, we inactivated calpain2 using CRISPR/Cas9 in epithelial MDCK cells. We show that depletion of calpain2 has multiple effects on cell morphology and function. Calpain2-depleted cells develop epithelial shape, however, they cover a smaller area, and cell clusters are more compact. Inactivation of calpain2 enhanced restoration of transepithelial electrical resistance after calcium switch, decreased cell migration, and delayed cell scattering induced by HGF/SF. In addition, calpain2 depletion prevented morphological changes induced by ERK2 overexpression. Interestingly, proteolysis of several calpain2 targets, including E-cadherin, I3-catenin, talin, FAK, and paxillin, was not discernibly affected by calpain2 depletion. Taken together, these data suggest that calpain2 regulates the stability of cell-cell and cell-substratum adhesions indirectly without affecting the proteolysis of these adhesion complexes. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2025 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0898656824002638?via%3Dihub
Počet záznamů: 1