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Structural characterization of wax esters using ultraviolet photodissociation mass spectrometry
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SYSNO ASEP 0587978 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Structural characterization of wax esters using ultraviolet photodissociation mass spectrometry Tvůrce(i) Kloudová, Barbora (UOCHB-X)
Vrkoslav, Vladimír (UOCHB-X) RID, ORCID
Polášek, Miroslav (UFCH-W) RID, ORCID
Bosáková, Z. (CZ)
Cvačka, Josef (UOCHB-X) RID, ORCIDZdroj.dok. Analytical and Bioanalytical Chemistry. - : Springer - ISSN 1618-2642
Roč. 416, č. 25 (2024), s. 5497-5512Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova wax ester ; UV photodissociation ; photochemistry ; double bond ; mass spectrometry Obor OECD Analytical chemistry CEP LX22NPO5104 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UOCHB-X - RVO:61388963 ; UFCH-W - RVO:61388955 UT WOS 001272308900001 EID SCOPUS 85198954455 DOI https://doi.org/10.1007/s00216-024-05434-2 Anotace Wax esters play critical roles in biological systems, serving functions from energy storage to chemical signaling. Their diversity is attributed to variations in alcohol and acyl chains, including their length, branching, and the stereochemistry of double bonds. Traditional analysis by mass spectrometry with collisional activations (CID, HCD) offers insights into acyl chain lengths and unsaturation level. Still, it falls short in pinpointing more nuanced structural features like the position of double bonds. As a solution, this study explores the application of 213-nm ultraviolet photodissociation (UVPD) for the detailed structural analysis of wax esters. It is shown that lithium adducts provide unique fragments as a result of Norrish and Norrish-Yang reactions at the ester moieties and photoinduced cleavages of double bonds. The product ions are useful for determining chain lengths and localizing double bonds. UVPD spectra of various wax esters are presented systematically, and the effect of activation time is discussed. The applicability of tandem mass spectrometry with UVPD is demonstrated for wax esters from natural sources. The UHPLC analysis of jojoba oil proves the compatibility of MS2 UVPD with the chromatography time scale, and a direct infusion is used to analyze wax esters from vernix caseosa. Data shows the potential of UVPD and its combination with CID or HCD in advancing our understanding of wax ester structures. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2025 Elektronická adresa https://doi.org/10.1007/s00216-024-05434-2
Počet záznamů: 1