Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting

Kalaninová, Zuzana; Fiala, Jan; Halada, Petr; Chmelík, Josef; Man, Petr; Kukačka, Zdeněk; Novák, Petr; Fojtík, Lukáš

doi:https://dx.doi.org/10.1021/acs.analchem.3c04161

Počet záznamů: 1

Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting

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SYSNO ASEP	0586459
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting
Tvůrce(i)	Fojtík, Lukáš (MBU-M)_ORCID Kalaninová, Zuzana (MBU-M)_{ORCID, RID} Fiala, Jan (MBU-M)_{ORCID, RID} Halada, Petr (MBU-M)_{RID, ORCID} Chmelík, Josef (MBU-M)_{RID, ORCID} Man, Petr (MBU-M)_{RID, ORCID} Kukačka, Zdeněk (MBU-M)_{RID, ORCID} Novák, Petr (MBU-M)_{RID, ORCID}
Zdroj.dok.	Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700 Roč. 96, č. 19 (2024), s. 7386-7393
Poč.str.	8 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	mass ; exchange ; identification ; complex ; proteins ; sequence
Obor OECD	Microbiology
CEP	EF18_046/0015974 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Výzkumná infrastruktura	CIISB II - 90127 - Masarykova univerzita
Způsob publikování	Open access
Institucionální podpora	MBU-M - RVO:61388971
UT WOS	001225426900001
EID SCOPUS	85192254871
DOI	https://doi.org/10.1021/acs.analchem.3c04161
Anotace	Covalent labeling in combination with mass spectrometry is a powerful approach used in structural biology to study protein structures, interactions, and dynamics. Recently, the toolbox of covalent labeling techniques has been expanded with fast fluoroalkylation of proteins (FFAP). FFAP is a novel radical labeling method that utilizes fluoroalkyl radicals generated from hypervalent Togni reagents for targeting aromatic residues. This report further demonstrates the benefits of FFAP as a new method for structural characterization of therapeutic antibodies and interaction interfaces of antigen-antibody complexes. The results obtained from human trastuzumab and its complex with human epidermal growth factor receptor 2 (HER2) correlate well with previously published structural data and demonstrate the potential of FFAP in structural biology.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2025
Elektronická adresa	https://pubs.acs.org/doi/10.1021/acs.analchem.3c04161

Počet záznamů: 1