Počet záznamů: 1
Expedient production of site specifically nucleobase-labelled or hypermodified RNA with engineered thermophilic DNA polymerases
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SYSNO ASEP 0585094 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Expedient production of site specifically nucleobase-labelled or hypermodified RNA with engineered thermophilic DNA polymerases Tvůrce(i) Brunderová, Mária (UOCHB-X) ORCID
Havlíček, Vojtěch (UOCHB-X)
Matyašovský, Ján (UOCHB-X) ORCID, RID
Pohl, Radek (UOCHB-X) RID, ORCID
Poštová Slavětínská, Lenka (UOCHB-X) RID
Krömer, Matouš (UOCHB-X) RID, ORCID
Hocek, Michal (UOCHB-X) RID, ORCIDČíslo článku 3054 Zdroj.dok. Nature Communications. - : Nature Publishing Group
Roč. 15, č. 1 (2024)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké CEP GX20-00885X GA ČR - Grantová agentura ČR EH22_008/0004575 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 001202403000013 EID SCOPUS 85189966748 DOI 10.1038/s41467-024-47444-9 Anotace Innovative approaches to controlled nucleobase-modified RNA synthesis are urgently needed to support RNA biology exploration and to synthesize potential RNA therapeutics. Here we present a strategy for enzymatic construction of nucleobase-modified RNA based on primer-dependent engineered thermophilic DNA polymerases – SFM4-3 and TGK. We demonstrate introduction of one or several different base-modified nucleotides in one strand including hypermodified RNA containing all four modified nucleotides bearing four different substituents, as well as strategy for primer segment removal. We also show facile site-specific or segmented introduction of fluorophores or other functional groups at defined positions in variety of RNA molecules, including structured or long mRNA. Intriguing translation efficacy of single-site modified mRNAs underscores the necessity to study isolated modifications placed at designer positions to disentangle their biological effects and enable development of improved mRNA therapeutics. Our toolbox paves the way for more precise dissecting RNA structures and functions, as well as for construction of diverse types of base-functionalized RNA for therapeutic applications and diagnostics. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2025 Elektronická adresa https://doi.org/10.1038/s41467-024-47444-9
Počet záznamů: 1