Počet záznamů: 1
Highly resolved genome assembly and comparative transcriptome profiling reveal genes related to developmental stages of tapeworm iLigula intestinalis/i
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SYSNO ASEP 0582763 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Highly resolved genome assembly and comparative transcriptome profiling reveal genes related to developmental stages of tapeworm iLigula intestinalis/i Tvůrce(i) Nazarizadeh Dehkordi, Masoud (BC-A) ORCID
Nováková, Milena (BC-A) ORCID
Drábková, M. (CZ)
Catchen, J. (US)
Olson, P. D. (GB)
Štefka, Jan (BC-A) RID, ORCIDCelkový počet autorů 6 Číslo článku 20232563 Zdroj.dok. Proceedings of the Royal Society B-Biological Sciences. - : Royal Society Publishing - ISSN 0962-8452
Roč. 291, č. 2015 (2024)Poč.str. 13 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova host-specificity ; life-cycle ; cestoda ; evolution ; diphyllobothriidea ; vitellogenesis ; duplication ; parasite ; database ; package ; Cestoda ; reference genome ; transcriptome ; life cycle ; parasite Vědní obor RIV EA - Morfologické obory a cytologie Obor OECD Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology CEP GA19-04676S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BC-A - RVO:60077344 UT WOS 001153940600006 EID SCOPUS 85183726188 DOI 10.1098/rspb.2023.2563 Anotace Ligula intestinalis (Cestoda: Diphyllobothriidae) is an emerging model organism for studies on parasite population biology and host-parasite interactions. However, a well-resolved genome and catalogue of its gene content has not been previously developed. Here, we present the first genome assembly of L. intestinalis, based on Oxford Nanopore Technologies, Illumina and Omni-C sequencing methodologies. We use transcriptome profiling to compare plerocercoid larvae and adult worms and identify differentially expressed genes (DEGs) associated with these life stages. The genome assembly is 775.3 mega (M)bp in size, with scaffold N50 value of 118 Mbp and encodes 27 256 predicted protein-coding sequences. Over 60% of the genome consists of repetitive sequences. Synteny analyses showed that the 10 largest scaffolds representing 75% of the genome display high correspondence to full chromosomes of cyclophyllidean tapeworms. Mapping RNA-seq data to the new reference genome, we identified 3922 differentially expressed genes in adults compared with plerocercoids. Gene ontology analyses revealed over-represented genes involved in reproductive development of the adult stage (e.g. sperm production) and significantly enriched DEGs associated with immune evasion of plerocercoids in their fish host. This study provides the first insights into the molecular biology of L. intestinalis and provides the most highly contiguous assembly to date of a diphyllobothriid tapeworm useful for population and comparative genomic investigations of parasitic flatworms. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2025 Elektronická adresa https://royalsocietypublishing.org/doi/10.1098/rspb.2023.2563
Počet záznamů: 1