Hematopoietic stem cells undergo a lymphoid to myeloid switch in early stages of emergency granulopoiesis

0579429 - ÚMG 2024 RIV US eng J - Článek v odborném periodiku
Vaníčková, Karolína - Miloševič, Mirko - Ribeiro Bas, Irina - Burocziová, Monika - Yokota, A. - Daněk, Petr - Grušanovič, Srdjan - Chilinski, M. - Plewczynski, D. - Rohlena, Jakub - Hirai, H. - Rohlenová, Kateřina - Alberich-Jorda, Meritxell
Hematopoietic stem cells undergo a lymphoid to myeloid switch in early stages of emergency granulopoiesis.
EMBO Journal. Roč. 42, č. 23 (2023), č. článku e113527. ISSN 0261-4189. E-ISSN 1460-2075
Grant CEP: GA MŠMT LX22NPO5102; GA ČR GA22-18300S
GRANT EU: European Commission(XE) 860002 - ENHPATHY
Grant ostatní: EMBO(DE) 5068-2022
Program: Installation Grant
Institucionální podpora: RVO:68378050 ; RVO:86652036
Klíčová slova: protein-c receptor * c/ebp-beta * progenitor cells * self-renewal * steady-state * gene * differentiation * signals * driven * cd201 * cd201 * emergency granulopoiesis * lymphoid-biased HSC * myeloid-biased HSC
Obor OECD: Cell biology; Cell biology (BTO-N)
Impakt faktor: 11.4, rok: 2022
Způsob publikování: Open access
https://www.embopress.org/doi/epdf/10.15252/embj.2023113527?src=getftr

Emergency granulopoiesis is the enhanced and accelerated production of granulocytes that occurs during acute infection. The contribution of hematopoietic stem cells (HSCs) to this process was reported, however, how HSCs participate in emergency granulopoiesis remains elusive. Here, using a mouse model of emergency granulopoiesis we observe transcriptional changes in HSCs as early as 4 h after lipopolysaccharide (LPS) administration. We observe that the HSC identity is changed towards a myeloid-biased HSC and show that CD201 is enriched in lymphoid-biased HSCs. While CD201 expression under steady-state conditions reveals a lymphoid bias, under emergency granulopoiesis loss of CD201 marks the lymphoid-to-myeloid transcriptional switch. Mechanistically, we determine that lymphoid-biased CD201+ HSCs act as a first response during emergency granulopoiesis due to direct sensing of LPS by TLR4 and downstream activation of NF-kappa Beta signaling. The myeloid-biased CD201- HSC population responds indirectly during an acute infection by sensing G-CSF, increasing STAT3 phosphorylation, and upregulating LAP/LAP* C/EBP beta isoforms. In conclusion, HSC subpopulations support early phases of emergency granulopoiesis due to their transcriptional rewiring from a lymphoid-biased to myeloid-biased population and thus establishing alternative paths to supply elevated numbers of granulocytes.
Trvalý link: https://hdl.handle.net/11104/0348238