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Are Histidine Kinases of Arbuscular Mycorrhizal Fungi Involved in the Response to Ethylene and Cytokinins?
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SYSNO ASEP 0577905 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Are Histidine Kinases of Arbuscular Mycorrhizal Fungi Involved in the Response to Ethylene and Cytokinins? Author(s) Mongès, A. (FR)
Yaakoub, H. (FR)
Bidon, B. (FR)
Glévarec, G. (FR)
Héricourt, F. (FR)
Carpin, S. (FR)
Chauderon, L. (FR)
Drašarová, Lenka (UEB-Q) ORCID
Spíchal, L. (CZ)
Binder, B. M. (US)
Papon, N. (FR)
Rochange, S. (FR)Number of authors 12 Source Title Molecular Plant-Microbe Interactions. - : American Phytopathological Society - ISSN 0894-0282
Roč. 36, č. 10 (2023), s. 656-665Number of pages 10 s. Language eng - English Country US - United States Keywords cytokinin ; ethylene ; mycorrhiza ; receptor ; Rhizophagus ; symbiosis OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 001089322500001 EID SCOPUS 85175357199 DOI https://doi.org/10.1094/MPMI-05-23-0056-R Annotation Signals are exchanged at all stages of the arbuscular mycorrhizal (AM) symbiosis between fungi and their host plants. Root-exuded strigolactones are well-known early symbiotic cues, but the role of other phytohormones as interkingdom signals has seldom been investigated. Here we focus on ethylene and cytokinins, for which candidate receptors have been identified in the genome of the AM fungus Rhizophagus irregularis. Ethylene is known from the literature to affect asymbiotic development of AM fungi, and in the present study, we found that three cytokinin forms could stimulate spore germination in R. irregularis. Heterologous complementation of a Saccharomyces cerevisiae mutant strain with the candidate ethylene receptor RiHHK6 suggested that this protein can sense and transduce an ethylene signal. Accordingly, its N-terminal domain expressed in Pichia pastoris displayed saturable binding to radiolabeled ethylene. Thus, RiHHK6 displays the expected characteristics of an ethylene receptor. In contrast, the candidate cytokinin receptor RiHHK7 did not complement the S. cerevisiae mutant strain or Medicago truncatula cytokinin receptor mutants and seemed unable to bind cytokinins, suggesting that another receptor is involved in the perception of these phytohormones. Taken together, our results support the hypothesis that AM fungi respond to a range of phytohormones and that these compounds bear multiple functions in the rhizosphere beyond their known roles as internal plant developmental regulators. Our analysis of two phytohormone receptor candidates also sheds new light on the possible perception mechanisms in AM fungi. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2024 Electronic address https://doi.org/10.1094/MPMI-05-23-0056-R
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