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Autologous Mesenchymal Stromal Cells Immobilized in Plasma-Based Hydrogel for the Repair of Articular Cartilage Defects in a Large Animal Model
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SYSNO ASEP 0577426 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Autologous Mesenchymal Stromal Cells Immobilized in Plasma-Based Hydrogel for the Repair of Articular Cartilage Defects in a Large Animal Model Tvůrce(i) Berounský, K. (CZ)
Vacková, I. (CZ)
Vištejnová, L. (CZ)
Malečková, A. (CZ)
Havránková, J. (CZ)
Klein, P. (CZ)
Kolinko, Y. (CZ)
Petrenko, Yuriy (UEM-P) RID, ORCID
Pražák, Š. (CZ)
Hanák, F. (CZ)
Přidal, J. (CZ)
Havlas, V. (CZ)Zdroj.dok. Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 72, č. 4 (2023), s. 485-495Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova Bone marrow mesenchymal stromal cells ; Scaffold Osteochondral defect ; Minipig ; Implantation Obor OECD Orthopaedics CEP NV19-06-00355 GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 UT WOS 001078316100007 EID SCOPUS 85173004424 DOI https://doi.org/10.33549/physiolres.935098 Anotace The treatment of cartilage defects in trauma injuries and degenerative diseases represents a challenge for orthopedists. Advanced mesenchymal stromal cell (MSC)-based therapies are currently of interest for the repair of damaged cartilage. However, an approved system for MSC delivery and maintenance in the defect is still missing. This study aimed to evaluate the effect of autologous porcine bone marrow MSCs anchored in a commercially available polyglycolic acid-hyaluronan scaffold (Chondrotissue (R)) using autologous blood plasma-based hydrogel in the repair of osteochondral defects in a large animal model. The osteochondral defects were induced in twenty-four minipigs with terminated skeletal growth. Eight animals were left untreated, eight were treated with Chondrotissue (R) and eight received Chondrotissue (R) loaded with MSCs. The animals were terminated 90 days after surgery. Macroscopically, the untreated defects were filled with newly formed tissue to a greater extent than in the other groups. The histological evaluations showed that the defects treated with Chondrotissue (R) and Chondrotissue (R) loaded with pBMSCs contained a higher amount of hyaline cartilage and a lower amount of connective tissue, while untreated defects contained a higher amount of connective tissue and a lower amount of hyaline cartilage. In addition, undifferentiated connective tissue was observed at the edges of defects receiving Chondrotissue (R) loaded with MSCs, which may indicate the extracellular matrix production by transplanted MSCs. The immunological analysis of the blood samples revealed no immune response activation by MSCs application. This study demonstrated the successful and safe immobilization of MSCs in commercially available scaffolds and defect sites for cartilage defect repair. Pracoviště Ústav experimentální medicíny Kontakt Arzuv Čaryjeva, arzuv.caryjeva@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2024 Elektronická adresa https://www.biomed.cas.cz/physiolres/pdf/2023/72_485.pdf
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