Počet záznamů: 1
Modified activities of macrophages’ deubiquitinating enzymes after Francisella infection
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SYSNO ASEP 0577416 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Modified activities of macrophages’ deubiquitinating enzymes after Francisella infection Tvůrce(i) Vozandychova, V. (CZ)
Řehulka, P. (CZ)
Hercík, Kamil (UOCHB-X) ORCID
Špidlová, P. (CZ)
Pavlík, P. (CZ)
Hanus, J. (CZ)
Hadravová, Romana (UOCHB-X) RID, ORCID
Stulík, J. (CZ)Číslo článku 1252827 Zdroj.dok. Frontiers in Immunology. - : Frontiers Media - ISSN 1664-3224
Roč. 14, September (2023)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Francisella ; deubiquitination ; DUBs ; exosomes ; extracellular vesicles ; USP10 ; UCH-l5 ; USP25 Obor OECD Microbiology Způsob publikování Open access Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 001085763100001 EID SCOPUS 85174231264 DOI https://doi.org/10.3389/fimmu.2023.1252827 Anotace Francisella tularensis influences several host molecular/signaling pathways during infection. Ubiquitination and deubiquitination are among the most important regulatory mechanisms and respectively occur through attachment or removal of the ubiquitin molecule. The process is necessary not only to mark molecules for degradation, but also, for example, to the activation of signaling pathways leading to pro-inflammatory host response. Many intracellular pathogens, including Francisella tularensis, have evolved mechanisms of modifying such host immune responses to escape degradation. Here, we describe that F. tularensis interferes with the host's ubiquitination system. We show increased total activity of deubiquitinating enzymes (DUBs) in human macrophages after infection, while confirm reduced enzymatic activities of two specific DUBs (USP10 and UCH-L5), and demonstrate increased activity of USP25. We further reveal the enrichment of these three enzymes in exosomes derived from F. tularensis-infected cells. The obtained results show the regulatory effect on ubiquitination mechanism in macrophages during F. tularensis infection. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2024 Elektronická adresa https://doi.org/10.3389/fimmu.2023.1252827
Počet záznamů: 1