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Cell lysis in the microfluidic chip: Towards single-molecule analysis in a free solution
- 1.0576907 - ÚIACH 2024 CZ eng A - Abstrakt
Křivánková, Jana - Hlaváček, Antonín - Weisová, Julie
Cell lysis in the microfluidic chip: Towards single-molecule analysis in a free solution.
12th International Conference Analytical Cytometry : Book of abstracts. Praha: AMCA, spol. s. r. o., 2023. s. 1-1.
[International Conference Analytical Cytometry /12./. 02.09.2023-05.09.2023, Praha]
Grant CEP: GA ČR(CZ) GA21-03156S
Institucionální podpora: RVO:68081715
Klíčová slova: droplet microfluidics * HeLa cells * detergent Triton X-100
Obor OECD: Analytical chemistry
https://www.conference.csac.cz/Amca-CSAC/media/content/2023/docs/Book_of_abstracts_CSAC_2023.pdf
Cell staining with Propidium iodide offered an effective tool for monitoring the progress of the cell lysis. Complete lysis in the microplate was reached with 0.01% (v/v) of the Triton X-100 after 10 minutes of passive detergent diffusion while 0.1% (v/v) of detergent was used for cell lysis in the PDMS droplet-based microfluidic chip. The proposed approach can be advantageously used for further cell manipulation and cell storage as well.
Trvalý link: https://hdl.handle.net/11104/0346293
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