Počet záznamů: 1
CHK1-CDC25A-CDK1 regulate cell cycle progression and protect genome integrity in early mouse embryos
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SYSNO ASEP 0576852 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název CHK1-CDC25A-CDK1 regulate cell cycle progression and protect genome integrity in early mouse embryos Tvůrce(i) Knoblochová, Lucie (UZFG-Y) ORCID
Ďuríček, Tomáš (UZFG-Y) ORCID
Vaškovičová, Michaela (UZFG-Y) ORCID
Zorzompokou, Chrysoula (UZFG-Y)
Rayová, Diana (UZFG-Y)
Ferencová, Ivana (UZFG-Y) ORCID
Baran, V. (SK)
Schultz, R. M. (US)
Hoffmann, E. R. (DK)
Drutovič, Dávid (UZFG-Y) ORCIDČíslo článku e56530 Zdroj.dok. Embo Reports - ISSN 1469-221X
Roč. 24, č. 10 (2023)Poč.str. 22 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova CDC25A phosphatase ; CDK1 kinase ; cell cycle regulation ; CHK1 kinase ; early mouse embryos Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology CEP GA20-27742S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 001119180600001 EID SCOPUS 85170519683 DOI https://doi.org/10.15252/embr.202256530 Anotace After fertilization, remodeling of the oocyte and sperm genomes is essential to convert these highly differentiated and transcriptionally quiescent cells into early cleavage-stage blastomeres that are transcriptionally active and totipotent. This developmental transition is accompanied by cell cycle adaptation, such as lengthening or shortening of the gap phases G1 and G2. However, regulation of these cell cycle changes is poorly understood, especially in mammals. Checkpoint kinase 1 (CHK1) is a protein kinase that regulates cell cycle progression in somatic cells. Here, we show that CHK1 regulates cell cycle progression in early mouse embryos by restraining CDK1 kinase activity due to CDC25A phosphatase degradation. CHK1 kinase also ensures the long G2 phase needed for genome activation and reprogramming gene expression in two-cell stage mouse embryos. Finally, Chk1 depletion leads to DNA damage and chromosome segregation errors that result in aneuploidy and infertility. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2024 Elektronická adresa https://www.embopress.org/doi/full/10.15252/embr.202256530
Počet záznamů: 1