Počet záznamů: 1
Astrocyte-like subpopulation of NG2 glia in the adult mouse cortex exhibits characteristics of neural progenitor cells
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SYSNO ASEP 0576841 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Astrocyte-like subpopulation of NG2 glia in the adult mouse cortex exhibits characteristics of neural progenitor cells Tvůrce(i) Janečková, Lucie (UMG-J)
Knotek, Tomáš (UEM-P)
Kriška, Ján (UEM-P) RID, ORCID
Heřmanová, Zuzana (UEM-P) ORCID
Kirdajová, Denisa (UEM-P) RID, ORCID
Kubovčiak, Jan (UMG-J)
Berková, Linda (UMG-J)
Turečková, Jana (UEM-P) ORCID
Garcia, Camacho, Sara (UEM-P)
Galušková, Kateřina (UMG-J)
Kolář, Michal (UMG-J) RID, ORCID
Anděrová, Miroslava (UEM-P) RID, ORCID
Kořínek, Vladimír (UMG-J) RIDCelkový počet autorů 13 Zdroj.dok. Glia. - : Wiley - ISSN 0894-1491
Roč. 72, č. 2 (2024), s. 245-273Poč.str. 29 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova chondroitin sulfate proteoglycan 4 ; focal cerebral ischemia ; neurogenesis ; NG2 glia heterogeneity ; oligodendrocyte precursor cell ; single-cell RNA sequencing Obor OECD Cell biology CEP GA21-24674S GA ČR - Grantová agentura ČR LM2018131 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2023050 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF18_046/0016045 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura Czech-BioImaging III - 90250 - Ústav molekulární genetiky AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 ; UEM-P - RVO:68378041 UT WOS 001074842800001 EID SCOPUS 85173489676 DOI https://doi.org/10.1002/glia.24471 Anotace Glial cells expressing neuron-glial antigen 2 (NG2), also known as oligodendrocyte progenitor cells (OPCs), play a critical role in maintaining brain health. However, their ability to differentiate after ischemic injury is poorly understood. The aim of this study was to investigate the properties and functions of NG2 glia in the ischemic brain. Using transgenic mice, we selectively labeled NG2-expressing cells and their progeny in both healthy brain and after focal cerebral ischemia (FCI). Using single-cell RNA sequencing, we classified the labeled glial cells into five distinct subpopulations based on their gene expression patterns. Additionally, we examined the membrane properties of these cells using the patch-clamp technique. Of the identified subpopulations, three were identified as OPCs, whereas the fourth subpopulation had characteristics indicative of cells likely to develop into oligodendrocytes. The fifth subpopulation of NG2 glia showed astrocytic markers and had similarities to neural progenitor cells. Interestingly, this subpopulation was present in both healthy and post-ischemic tissue, however, its gene expression profile changed after ischemia, with increased numbers of genes related to neurogenesis. Immunohistochemical analysis confirmed the temporal expression of neurogenic genes and showed an increased presence of NG2 cells positive for Purkinje cell protein-4 at the periphery of the ischemic lesion 12 days after FCI, as well as NeuN-positive NG2 cells 28 and 60 days after injury. These results suggest the potential development of neuron-like cells arising from NG2 glia in the ischemic tissue. Our study provides insights into the plasticity of NG2 glia and their capacity for neurogenesis after stroke. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2025 Elektronická adresa https://onlinelibrary.wiley.com/doi/10.1002/glia.24471
Počet záznamů: 1