Počet záznamů: 1
Hormone profiling and the root proteome analysis of itpk1 mutant seedlings of barley (Hordeum vulgare) during the red-light induced photomorphogenesis
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SYSNO ASEP 0575624 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Hormone profiling and the root proteome analysis of itpk1 mutant seedlings of barley (Hordeum vulgare) during the red-light induced photomorphogenesis Tvůrce(i) Vlčko, Tomáš (UEB-Q) ORCID
Tarkowská, Danuše (UEB-Q) RID, ORCID
Široká, Jitka (UEB-Q) ORCID
Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Simerský, R. (CZ)
Chamrád, Ivo (UEB-Q) ORCID
Lenobel, René (UEB-Q) ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Ohnoutková, Ludmila (UEB-Q) RID, ORCIDCelkový počet autorů 9 Číslo článku 105428 Zdroj.dok. Environmental and Experimental Botany. - : Elsevier - ISSN 0098-8472
Roč. 213, SEP (2023)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Barley ; Inositol phosphate ; itpk ; Phytohormone ; Red light Obor OECD Plant sciences, botany CEP EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 001032342500001 EID SCOPUS 85163796936 DOI 10.1016/j.envexpbot.2023.105428 Anotace Inositol phosphates represent crucial primary metabolites, known as phosphate storage molecules or molecules enabling protein interactions. Inositol phosphates and their pyrophosphate forms direct the assembly of the COP9 signalosome and Cullin Ring Ligase complexes. Thus, inositol phosphates participate on protein degradation, which is a means of signal transduction for several plant hormone classes and light signaling cascade. In plants, production of inositol phosphates is maintained by several inositol phosphate kinases including the group of inositol-1,3,4-trisphosphate 5/6 kinases (ITPKs). The members of ITPKs connect plant phosphate status with light perception and hormonal balance. Photomorphogenesis in red light was investigated using allelic variants of barley Hvitpk1 mutants produced by gene editing. Barley ITPK promoter regions, conserved protein motifs, and expression profiles were investigated to characterize their evolutionary diversification and transcriptional regulation. We focused on ITPK1 relation to auxins, cytokinins, gibberellins, jasmonates, abscisic acid, and salicylic acid in red light-induced photomorphogenesis. Global proteome analyses was conducted in the mutant and wild-type (WT) seedlings grown under continuous red light. Our results demonstrate that different allelic variants of itpk1 mutants manifested defects in producing important phytohormones with consequences for root growth. Mutations in itpk1 affected hormone biosynthesis and metabolite, while proteome analysis revealed significant differences in seedling root proteomes between mutant lines. Based on our results and published data, a model of ITPK activity is proposed depicting them as regulators of hormone signal transduction at the level of protein degradation. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2024 Elektronická adresa https://doi.org/10.1016/j.envexpbot.2023.105428
Počet záznamů: 1