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Sulfonated polystyrenes: pH and Mg2+-insensitive amphiphilic copolymers for detergent-free membrane protein isolation
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SYSNO ASEP 0575486 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Sulfonated polystyrenes: pH and Mg2+-insensitive amphiphilic copolymers for detergent-free membrane protein isolation Tvůrce(i) Janata, Miroslav (UMCH-V) RID
Gupta, Sachin (UMCH-V) RID, ORCID
Čadová, Eva (UMCH-V) RID
Angelisová, Pavla (UMG-J) RID
Krishnarjuna, B. (US)
Ramamoorthy, A. (US)
Hořejší, Václav (UMG-J) RID
Raus, Vladimír (UMCH-V) RID, ORCIDČíslo článku 112412 Zdroj.dok. European Polymer Journal. - : Elsevier - ISSN 0014-3057
Roč. 198, 17 October (2023)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova amphiphilic copolymer ; sulfonated polystyrene ; membrane protein Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science Vědní obor RIV – spolupráce Ústav molekulární genetiky - Genetika a molekulární biologie CEP GA19-04047S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 ; UMG-J - RVO:68378050 UT WOS 001074790900001 EID SCOPUS 85171437852 DOI https://doi.org/10.1016/j.eurpolymj.2023.112412 Anotace Amphiphilic polymers are increasingly applied in the detergent-free isolation and functional studies of membrane proteins. However, the carboxylate group present in the structure of many popular variants, such as styrene-maleic acid (SMA) copolymers, brings limitations in terms of polymer sensitivity to precipitation at acidic pH or in the presence of divalent metal cations. Herein, we addressed this problem by replacing carboxylate with the more acidic sulfonate groups. To this end, we synthesized a library of amphiphilic poly[styrene-co-(sodium 4-styrene sulfonate)] copolymers (termed SSS), differing in their molecular weight and overall polarity. Using model cell membranes (Jurkat), we identified two copolymer compositions (SSS-L30 and SSS-L36) that solubilized membranes to an extent similar to SMA. Interestingly, the density gradient ultracentrifugation/SDS-PAGE/Western blotting analysis of cell lysates revealed a distribution of studied membrane proteins in the gradient fractions that was different than for SMA-solubilized membranes. Importantly, unlike SMA, the SSS copolymers remained soluble at low pH and in the presence of Mg2+ ions. Additionally, the solubilization of DMPC liposomes by the lead materials was studied by turbidimetry, DLS, SEC, and high-resolution NMR, revealing, for SSS-L36, the formation of stable particles (nanodiscs), facilitated by the direct hydrophobic interaction of the copolymer phenyls with lipid acyl chains. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2024 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0014305723005955?via%3Dihub
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