Počet záznamů: 1
Genosensing on a 3D-printed nanocarbon electrode
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SYSNO ASEP 0575169 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Genosensing on a 3D-printed nanocarbon electrode Tvůrce(i) Jyoti, A. (CZ)
Fojta, Miroslav (BFU-R) RID, ORCID
Hermanová, Monika (BFU-R) ORCID
Pivoňková, Hana (BFU-R) ORCID, RID
Alduhaish, O. (SA)
Pumera, M. (CZ)Celkový počet autorů 6 Číslo článku 107508 Zdroj.dok. Electrochemistry Communications. - : Elsevier - ISSN 1388-2481
Roč. 151, JUN 2023 (2023)Poč.str. 8 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova 3D-printed nanocarbon electrode ; DNA hybridization ; Electrochemical analysis ; Additive manufacturing Vědní obor RIV CG - Elektrochemie Obor OECD Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) CEP GA20-03187S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 001055116600001 DOI https://doi.org/10.1016/j.elecom.2023.107508 Anotace In this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization. The approach takes advantage of a facile procedure based on adsorption of target DNA on the electrode surface followed by hybridization with a biotinylated probe and binding of streptavidin-alkaline phosphatase conjugate. The alkaline phosphatase converts 1-naphthyl phosphate in the background electrolyte into electrochemically oxidizable 1-naphthol, which is subsequently detected using linear sweep voltammetry. The preparation, characterization, and analytical performance of the 3DnCes are reported. The results show the applicability of such 3DnCes in detection of target DNA hybridization specifically with the complementary biotinylated probe, and indicate the potential of 3D printed electrodes for use in various bioanalytical approaches. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2024 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S1388248123000826?via%3Dihub
Počet záznamů: 1