Počet záznamů: 1
Antiviral activity of singlet oxygen-photogenerating perylene compounds against SARS-CoV-2: Interaction with the viral envelope and photodynamic virion inactivation
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SYSNO ASEP 0574493 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Antiviral activity of singlet oxygen-photogenerating perylene compounds against SARS-CoV-2: Interaction with the viral envelope and photodynamic virion inactivation Tvůrce(i) Straková, Petra (BC-A) ORCID, RID
Bednář, Petr (BC-A) ORCID
Kotouček, J. (CZ)
Holoubek, Jiří (BC-A) RID
Fořtová, A. (CZ)
Svoboda, Pavel (BC-A) ORCID
Štefánik, M. (CZ)
Huvarová, I. (CZ)
Šimečková, P. (CZ)
Mašek, J. (CZ)
Gvozdev, D.A. (RU)
Mikhnovets, I.E. (RU)
Chistov, A.A. (RU)
Nikitin, T.D. (RU)
Krasilnikov, M.S. (RU)
Ustinov, A. (RU)
Alferova, V.A. (RU)
Korshun, V.A. (RU)
Růžek, Daniel (BC-A) RID, ORCID
Eyer, Luděk (BC-A) RID, ORCIDCelkový počet autorů 20 Číslo článku 199158 Zdroj.dok. Virus Research. - : Elsevier - ISSN 0168-1702
Roč. 334, SEPT (2023)Poč.str. 21 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova SARS-CoV-2 ; Perylene-related compound ; Antiviral activity ; Membrane ; Liposome ; Photodynamic inactivation Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Virology CEP LX22NPO5103 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 001037067400001 EID SCOPUS 85164501348 DOI 10.1016/j.virusres.2023.199158 Anotace The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has prompted great interest in novel broad-spectrum antivirals, including perylene-related compounds. In the present study, we performed a structure-activity relationship analysis of a series of per-ylene derivatives, which comprised a large planar perylene residue, and structurally divergent polar groups connected to the perylene core by a rigid ethynyl or thiophene linker. Most of the tested compounds did not exhibit significant cytotoxicity towards multiple cell types susceptible to SARS-CoV-2 infection, and did not change the expressions of cellular stress-related genes under normal light conditions. These compounds showed nanomolar or sub-micromolar dose-dependent anti-SARS-CoV-2 activity, and also suppressed the in vitro repli-cation of feline coronavirus (FCoV), also termed feline infectious peritonitis virus (FIPV). Perylene compounds exhibited high affinity for liposomal and cellular membranes, and efficiently intercalated into the envelopes of SARS-CoV-2 virions, thereby blocking the viral-cell fusion machinery. Furthermore, the studied compounds were demonstrated to be potent photosensitizers, generating reactive oxygen species (ROS), and their anti-SARS-CoV-2 activities were considerably enhanced after irradiation with blue light. Our results indicated that photosen-sitization is the major mechanism underlying the anti-SARS-CoV-2 activity of perylene derivatives, with these compounds completely losing their antiviral potency under red light. Overall, perylene-based compounds are broad-spectrum antivirals against multiple enveloped viruses, with antiviral action based on light-induced photochemical damage (ROS-mediated, likely singlet oxygen-mediated), causing impairment of viral mem-brane rheology. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2024 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S016817022300120X?via%3Dihub
Počet záznamů: 1