Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding

0574364 - FGÚ 2024 RIV CH eng J - Článek v odborném periodiku
Horáková, Olga - Sistilli, Gabriella - Kalendová, Veronika - Bardová, Kristina - Mitrović, Marko - Čajka, Tomáš - Irodenko, Ilaria - Janovská, Petra - Lackner, K. - Kopecký, Jan - Rossmeisl, Martin
Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding.
Frontiers in Endocrinology. Roč. 14, Jul 12 (2023), č. článku 1205703. ISSN 1664-2392. E-ISSN 1664-2392
Grant CEP: GA MZd(CZ) NU21-01-00469; GA MŠMT(CZ) LX22NPO5104
GRANT EU: European Commission(XE) 722619 - Foie Gras
Institucionální podpora: RVO:67985823
Klíčová slova: non-alcoholic fatty liver disease * liver steatosis * NASH * C57BL/6N mice * obesity * thermoneutrality * metabolomics * de novo lipogenesis
Obor OECD: Endocrinology and metabolism (including diabetes, hormones)
Impakt faktor: 5.2, rok: 2022
Způsob publikování: Open access
https://doi.org/10.3389/fendo.2023.1205703

Introduction: Non-alcoholic fatty liver disease (NAFLD) can progress to more severe stages, such as steatohepatitis and fibrosis. Thermoneutral housing together with high-fat diet promoted NAFLD progression in C57BL/6J mice. Due to possible differences in steatohepatitis development between different C57BL/6 substrains, we examined how thermoneutrality affects NAFLD progression in C57BL/6N mice.
Methods: Male mice were fed standard or high-fat diet for 24 weeks and housed under standard (22°C) or thermoneutral (30°C) conditions.
Results: High-fat feeding promoted weight gain and hepatic steatosis, but the effect of thermoneutral environment was not evident. Liver expression of inflammatory markers was increased, with a modest and inconsistent effect of thermoneutral housing, however, histological scores of inflammation and fibrosis were generally low (<1.0), regardless of ambient temperature. In standard diet-fed mice, thermoneutrality increased weight gain, adiposity, and hepatic steatosis, accompanied by elevated de novo lipogenesis and changes in liver metabolome characterized by complex decreases in phospholipids and metabolites involved in urea cycle and oxidative stress defense.
Conclusion: Thermoneutrality appears to promote NAFLD-associated phenotypes depending on the C57BL/6 substrain and/or the amount of dietary fat.

Trvalý link: https://hdl.handle.net/11104/0344701