Počet záznamů: 1
RAD51 and RAD51B Play Diverse Roles in the Repair of DNA Double Strand Breaks in Physcomitrium patens
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SYSNO ASEP 0571815 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název RAD51 and RAD51B Play Diverse Roles in the Repair of DNA Double Strand Breaks in Physcomitrium patens Tvůrce(i) Angelis, Karel (UEB-Q) RID, ORCID
Záveská Drábková, Lenka (UEB-Q) ORCID
Vágnerová, Radka (UEB-Q) ORCID
Holá, Marcela (UEB-Q) ORCIDCelkový počet autorů 4 Číslo článku 305 Zdroj.dok. Genes. - : MDPI
Roč. 14, č. 2 (2023)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova bleomycin ; comet assay ; DNA double-strand break (DSB) ; evolutionary divergence ; homologous recombination (HR) ; non-homologous end-joining (NHEJ) ; Physcomitrella ; rDNA ; repair kinetic Obor OECD Plant sciences, botany CEP GA20-05095S GA ČR - Grantová agentura ČR LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000939143100001 EID SCOPUS 85148880139 DOI 10.3390/genes14020305 Anotace RAD51 is involved in finding and invading homologous DNA sequences for accurate homologous recombination (HR). Its paralogs have evolved to regulate and promote RAD51 functions. The efficient gene targeting and high HR rates are unique in plants only in the moss Physcomitrium patens (P. patens). In addition to two functionally equivalent RAD51 genes (RAD1-1 and RAD51-2), other RAD51 paralogues were also identified in P. patens. For elucidation of RAD51’s involvement during DSB repair, two knockout lines were constructed, one mutated in both RAD51 genes (Pprad51-1-2) and the second with mutated RAD51B gene (Pprad51B). Both lines are equally hypersensitive to bleomycin, in contrast to their very different DSB repair efficiency. Whereas DSB repair in Pprad51-1-2 is even faster than in WT, in Pprad51B, it is slow, particularly during the second phase of repair kinetic. We interpret these results as PpRAD51-1 and2 being true functional homologs of ancestral RAD51 involved in the homology search during HR. Absence of RAD51 redirects DSB repair to the fast NHEJ pathway and leads to a reduced 5S and 18S rDNA copy number. The exact role of the RAD51B paralog remains unclear, though it is important in damage recognition and orchestrating HR response. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2024 Elektronická adresa https://doi.org/10.3390/genes14020305
Počet záznamů: 1