Počet záznamů: 1
Dysregulation of hypoxia-inducible factor 1 alpha in the sympathetic nervous system accelerates diabetic cardiomyopathy
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SYSNO ASEP 0571541 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Dysregulation of hypoxia-inducible factor 1 alpha in the sympathetic nervous system accelerates diabetic cardiomyopathy Tvůrce(i) Hrabalová, Petra (BTO-N) RID
Bohuslavová, Romana (BTO-N) RID
Matějková, Kateřina (BTO-N)
Papoušek, František (FGU-C)
Sedmera, David (FGU-C) RID, ORCID, SAI
Abaffy, Pavel (BTO-N)
Kolář, František (FGU-C) RID, ORCID, SAI
Pavlínková, Gabriela (BTO-N) RID, ORCIDCelkový počet autorů 8 Číslo článku 88 Zdroj.dok. Cardiovascular Diabetology. - : BioMed Central
Roč. 22, č. 1 (2023)Poč.str. 21 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Cardiac function ; Inflammation ; Diabetic cardiomyopathy ; Collagen deposition ; Sympathetic neurons Vědní obor RIV FB - Endokrinologie, diabetologie, metabolizmus, výživa Obor OECD Endocrinology and metabolism (including diabetes, hormones) CEP LX22NPO5104 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA21-03847S GA ČR - Grantová agentura ČR EF18_046/0016045 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CCP II - 90126 - Ústav molekulární genetiky AV ČR, v. v. i.
Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i.Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 ; FGU-C - RVO:67985823 UT WOS 000971837600002 EID SCOPUS 85152863980 DOI 10.1186/s12933-023-01824-5 Anotace BackgroundAn altered sympathetic nervous system is implicated in many cardiac pathologies, ranging from sudden infant death syndrome to common diseases of adulthood such as hypertension, myocardial ischemia, cardiac arrhythmias, myocardial infarction, and heart failure. Although the mechanisms responsible for disruption of this well-organized system are the subject of intensive investigations, the exact processes controlling the cardiac sympathetic nervous system are still not fully understood. A conditional knockout of the Hif1a gene was reported to affect the development of sympathetic ganglia and sympathetic innervation of the heart. This study characterized how the combination of HIF-1 alpha deficiency and streptozotocin (STZ)-induced diabetes affects the cardiac sympathetic nervous system and heart function of adult animals.MethodsMolecular characteristics of Hif1a deficient sympathetic neurons were identified by RNA sequencing. Diabetes was induced in Hif1a knockout and control mice by low doses of STZ treatment. Heart function was assessed by echocardiography. Mechanisms involved in adverse structural remodeling of the myocardium, i.e. advanced glycation end products, fibrosis, cell death, and inflammation, was assessed by immunohistological analyses.ResultsWe demonstrated that the deletion of Hif1a alters the transcriptome of sympathetic neurons, and that diabetic mice with the Hif1a-deficient sympathetic system have significant systolic dysfunction, worsened cardiac sympathetic innervation, and structural remodeling of the myocardium.ConclusionsWe provide evidence that the combination of diabetes and the Hif1a deficient sympathetic nervous system results in compromised cardiac performance and accelerated adverse myocardial remodeling, associated with the progression of diabetic cardiomyopathy. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2024 Elektronická adresa https://cardiab.biomedcentral.com/articles/10.1186/s12933-023-01824-5
Počet záznamů: 1