Počet záznamů: 1
Singlet oxygen in vivo: it is all about intensity - part 2
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SYSNO ASEP 0571287 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Singlet oxygen in vivo: it is all about intensity - part 2 Tvůrce(i) Hackbarth, S. (DE)
Gao, S. (JP)
Šubr, Vladimír (UMCH-V) RID, ORCID
Lin, L. (DE)
Pohl, J. (DE)
Etrych, Tomáš (UMCH-V) RID, ORCID
Fang, J. (JP)Číslo článku 781 Zdroj.dok. Journal of Personalized Medicine. - : MDPI
Roč. 13, č. 5 (2023)Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova photodynamic therapy ; singlet oxygen ; time-resolved phosphorescence Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science CEP NU21-08-00280 GA MZd - Ministerstvo zdravotnictví LX22NPO5102 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMCH-V - RVO:61389013 UT WOS 001020900500001 EID SCOPUS 85160331617 DOI https://doi.org/10.3390/jpm13050781 Anotace Recently, we reported induced anoxia as a limiting factor for photodynamic tumor therapy (PDT). This effect occurs in vivo if the amount of generated singlet oxygen that undergoes chemical reactions with cellular components exceeds the local oxygen supply. The amount of generated singlet oxygen depends mainly on photosensitizer (PS) accumulation, efficiency, and illumination intensity. With illumination intensities above a certain threshold, singlet oxygen is limited to the blood vessel and the nearest vicinity. Lower intensities allow singlet oxygen generation also in tissue which is a few cell layers away from the vessels. While all experiments so far were limited to light intensities above this threshold, we report experimental results for intensities at both sides of the threshold for the first time, giving proof for the described model. Using time-resolved optical detection in NIR, we demonstrate characteristic, illumination intensity-dependent changes in signal kinetics of singlet oxygen and photosensitizer phosphorescence in vivo. The described analysis allows for better optimization and coordination of PDT drugs and treatment, as well as new diagnostic methods based on gated PS phosphorescence, for which we report a first in vivo feasibility test.
Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2024 Elektronická adresa https://www.mdpi.com/2075-4426/13/5/781
Počet záznamů: 1