Počet záznamů: 1
Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration
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SYSNO ASEP 0571228 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration Tvůrce(i) Fedorová, V. (CZ)
Pospíšilová, V. (CZ)
Váňová, T. (CZ)
Amruz Černá, K. (CZ)
Abaffy, Pavel (BTO-N)
Sedmík, J. (CZ)
Raška, J. (CZ)
Vochyánova, S. (CZ)
Matúšová, Zuzana (BTO-N)
Houserová, Jana (UMG-J)
Valihrach, Lukáš (BTO-N) RID, ORCID
Hodný, Zdeněk (UMG-J) RID
Bohaciaková, D. (CZ)Celkový počet autorů 13 Zdroj.dok. Molecular Oncology. - : Wiley - ISSN 1574-7891
Roč. 17, č. 4 (2023), s. 647-663Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova cerebral organoids ; glico ; glioblastoma ; induced pluripotent stem cells Obor OECD Oncology CEP GA20-15728S GA ČR - Grantová agentura ČR LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i.
RECETOX RI - 90121 - Masarykova univerzitaZpůsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 ; UMG-J - RVO:68378050 UT WOS 000934781400001 EID SCOPUS 85148460718 DOI 10.1002/1878-0261.13389 Anotace It is currently challenging to adequately model the growth and migration of glioblastoma using two-dimensional (2D) in vitro culture systems as they quickly lose the original, patient-specific identity and heterogeneity. However, with the advent of three-dimensional (3D) cell cultures and human-induced pluripotent stem cell (iPSC)-derived cerebral organoids (COs), studies demonstrate that the glioblastoma-CO (GLICO) coculture model helps to preserve the phenotype of the patient-specific tissue. Here, we aimed to set up such a model using mature COs and develop a pipeline for subsequent analysis of cocultured glioblastoma. Our data demonstrate that the growth and migration of the glioblastoma cell line within the mature COs are significantly increased in the presence of extracellular matrix proteins, shortening the time needed for glioblastoma to initiate migration. We also describe in detail the method for the visualization and quantification of these migrating cells within the GLICO model. Lastly, we show that this coculture model (and the human brain-like microenvironment) can significantly transform the gene expression profile of the established U87 glioblastoma cell line into proneural and classical glioblastoma cell types. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2024 Elektronická adresa https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1878-0261.13389
Počet záznamů: 1