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PLCL/PCL dressings with platelet lysate and growth factors embedded in fibrin for chronic wound regeneration
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SYSNO ASEP 0568881 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název PLCL/PCL dressings with platelet lysate and growth factors embedded in fibrin for chronic wound regeneration Tvůrce(i) Táborská, Johanka (UMCH-V)
Blanquer, Andreu (FGU-C) RID, ORCID
Brynda, Eduard (UMCH-V) RID
Filová, Elena (FGU-C) RID, ORCID
Stiborová, Lenka (UMCH-V)
Jenčová, V. (CZ)
Havlíčková, K. (CZ)
Riedelová, Zuzana (UMCH-V) ORCID
Riedel, Tomáš (UMCH-V) RID, ORCIDZdroj.dok. International Journal of Nanomedicine. - : Dove Medical Press - ISSN 1178-2013
Roč. 18, 3 February (2023), s. 595-610Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. NZ - Nový Zéland Klíč. slova human platelet lysate ; diabetic ulcer ; fibrin coating Vědní obor RIV FB - Endokrinologie, diabetologie, metabolizmus, výživa Obor OECD Endocrinology and metabolism (including diabetes, hormones) CEP NV18-01-00332 GA MZd - Ministerstvo zdravotnictví LX22NPO5104 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMCH-V - RVO:61389013 ; FGU-C - RVO:67985823 UT WOS 000928064400001 EID SCOPUS 85147762343 DOI https://doi.org/10.2147/IJN.S393890 Anotace The formation of diabetic ulcers (DU) is a common complication for diabetic patients resulting in serious chronic wounds. There is therefore, an urgent need for complex treatment of this problem. This study examines a bioactive wound dressing of a biodegradable electrospun nanofibrous blend of poly(L-lactide-co-ϵ-caprolactone) and poly(ϵ-caprolactone) (PLCL/PCL) covered by a thin fibrin layer for sustained delivery of bioactive molecules. Electrospun PLCL/PCL nanofibers were coated with fibrin-based coating prepared by a controlled technique and enriched with human platelet lysate (hPL), fibroblast growth factor 2 (FGF), and vascular endothelial growth factor (VEGF). The coating was characterized by scanning electron microscopy and fluorescent microscopy. Protein content and its release rate and the effect on human saphenous vein endothelial cells (HSVEC) were evaluated. The highest protein amount is achieved by the coating of PLCL/PCL with a fibrin mesh containing 20% v/v hPL (NF20). The fibrin coating serves as an excellent scaffold to accumulate bioactive molecules from hPL such as PDGF-BB, fibronectin (Fn), and α-2 antiplasmin. The NF20 coating shows both fast and a sustained release of the attached bioactive molecules (Fn, VEGF, FGF). The dressing significantly increases the viability of human saphenous vein endothelial cells (HSVECs) cultivated on a collagen-based wound model. The exogenous addition of FGF and VEGF during the coating procedure further increases the HSVECs viability. In addition, the presence of α-2 antiplasmin significantly stabilizes the fibrin mesh and prevents its cleavage by plasmin. The NF20 coating supplemented with FGF and VEGF provides a promising wound dressing for the complex treatment of DU. The incorporation of various bioactive molecules from hPL and growth factors has great potential to support the healing processes by providing appropriate stimuli in the chronic wound. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2024 Elektronická adresa https://www.dovepress.com/plclpcl-dressings-with-platelet-lysate-and-growth-factors-embedded-in--peer-reviewed-fulltext-article-IJN
Počet záznamů: 1