Počet záznamů: 1
Lab-on-chip microscope platform for electro-manipulation of a dense microtubules network
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SYSNO ASEP 0564452 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Lab-on-chip microscope platform for electro-manipulation of a dense microtubules network Tvůrce(i) Havelka, D. (CZ)
Zhernov, Ilia (BTO-N) ORCID
Teplan, M. (SK)
Lánský, Zdeněk (BTO-N) ORCID, RID
Chafai, Djamel Eddine (FGU-C) ORCID
Cifra, M. (CZ)Číslo článku 2462 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 12, č. 1 (2022)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova kinesin motors ; tubulin ; migration ; alignment ; cells Obor OECD Neurosciences (including psychophysiology Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 ; BTO-N - RVO:86652036 UT WOS 000755212600015 EID SCOPUS 85124615232 DOI https://doi.org/10.1038/s41598-022-06255-y Anotace Pulsed electric field (PEF) technology is promising for the manipulation of biomolecular components and has potential applications in biomedicine and bionanotechnology. Microtubules, nanoscopic tubular structures self-assembled from protein tubulin, serve as important components in basic cellular processes as well as in engineered biomolecular nanosystems. Recent studies in cell-based models have demonstrated that PEF affects the cytoskeleton, including microtubules. However, the direct effects of PEF on microtubules are not clear. In this work, we developed a lab-on-a-chip platform integrated with a total internal reflection fluorescence microscope system to elucidate the PEF effects on a microtubules network mimicking the cell-like density of microtubules. The designed platform enables the delivery of short (microsecond-scale), high-field-strength (<= 25 kV/cm) electric pulses far from the electrode/electrolyte interface. We showed that microsecond PEF is capable of overcoming the non-covalent microtubule bonding force to the substrate and translocating the microtubules. This microsecond PEF effect combined with macromolecular crowding led to aggregation of microtubules. Our results expand the toolbox of bioelectronics technologies and electromagnetic tools for the manipulation of biomolecular nanoscopic systems and contribute to the understanding of microsecond PEF effects on a microtubule cytoskeleton. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2023 Elektronická adresa https://doi.org/10.1038/s41598-022-06255-y
Počet záznamů: 1