Počet záznamů: 1
Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses
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SYSNO ASEP 0562612 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses Tvůrce(i) Konečná, Klára (BFU-R) ORCID
Kilar, A. (CZ)
Kovacikova, P. (CZ)
Fajkus, Jiří (BFU-R) RID, ORCID
Sýkorová, Eva (BFU-R) RID, ORCID
Fojtová, M. (CZ)Celkový počet autorů 6 Číslo článku 111453 Zdroj.dok. Plant Science. - : Elsevier - ISSN 0168-9452
Roč. 325, DEC 2022 (2022)Poč.str. 16 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. IE - Irsko Klíč. slova arm ; Arabidopsis thaliana ; Armadillo ; ?-catenin-like repeat ; RNAseq ; Stress response ; Telomerase Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA21-28265S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BFU-R - RVO:68081707 UT WOS 000862382300002 EID SCOPUS 85138552413 DOI 10.1016/j.plantsci.2022.111453 Anotace ARM was identified previously as an interaction partner of the telomerase protein subunit (TERT) in Arabidopsis thaliana. To investigate the interconnection between ARM and telomerase and to identify ARM cellular functions, we analyzed a set of arm mutant lines and arm/tert double mutants. Telomere length was not affected in arm single mutant plants, in contrast to double mutants. In the second generation of homozygous arm-1/tert double mutants following the heterozygous state during the double mutant construction, telomeres shortened dramat-ically, even below levels in tert plants displaying severe morphological defects. Intriguingly, homozygous arm-1/ tert double mutants with short telomeres grew without obvious phenotypic changes for next two generations. Then, in agreement with the onset of phenotypic changes in tert, morphological defects were timed to the 5th arm-1/tert homozygous generation. RNAseq analyses of arm-1/tert and respective single mutants displayed markedly overlapping sets of differentially expressed genes in arm-1/tert double mutant and arm-1 single mutant lines, indicating a dominant effect of the ARM mutation. RNAseq data further implied ARM involvement in circadian rhythms, responses to drugs and to biotic and abiotic stimuli. In agreement with it, we observed sensitivity of arm-1 single mutant to the heat stress during germination. Altogether, our results suggest ARM involvement in crucial cellular processes without evidencing its role in the telomerase canonical function. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2023 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0168945222002783?via%3Dihub
Počet záznamů: 1