Počet záznamů: 1
TALE protein mediated overexpression of embryogenesis related marker genes in wheat microspores
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SYSNO ASEP 0559502 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název TALE protein mediated overexpression of embryogenesis related marker genes in wheat microspores Tvůrce(i) Sinha, Rakesh Kumar (BC-A) RID
Jiang, F. (CA)
Eudes, F. (CA)Celkový počet autorů 3 Zdroj.dok. South African Journal of Botany. - : Elsevier - ISSN 0254-6299
Roč. 138, May 01 (2021), s. 50-56Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. ZA - Jižní Afrika Klíč. slova Microspore: RKD ECA1 TPD1 ; TALE protein ; Embryogenesis Vědní obor RIV BO - Biofyzika Obor OECD Biophysics CEP EF15_003/0000336 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000648693200004 EID SCOPUS 85099249828 DOI 10.1016/j.sajb.2020.12.004 Anotace Stress treatment of microspore embryogenesis leads to the reprogramming of male gametophyte development pathways, which generates haploid green plants. However, low green plant production efficiency is a major hindrance to the adoption of the double haploid (DH) production platform for wheat breeding. The aim of the current study was to induce the expression of microspore embryogenesis-associated genes by overexpressing the RKD transcription factor using Transcription activator-like effector (TALE) proteins. The TALE protein can modulate the gene expression by recognizing and binding host plant promoter sequences. Taking into consideration, we have cloned and analysed RKD promoters from wheat and triticale and used them for the custom synthesis of TaRKD-TALE protein. The isolated and purified protein was conjugated with R9 (Cys (Npys)-(D-Arg)9) cargo peptides and successfully transduced into wheat microspores. The expression of early-culture abundant (ECA1), RWP-RK domain-containing proteins (RKD1) and Tapetum determinant 1 (TPD1) marker genes in wheat linked to microspore embryogenesis were studied. The expression of all three (ECA1, TPD1 and RKD1) embryogenesis-related marker genes in the TALE transduced microspore was significantly higher than in the control. Microspore culture transduced by R9-TaRKD-TALE protein yielded a significantly higher number of embryo-like structures (ELS) and the total green plants in wheat cultivars AC Fielder. Conversely, the microspore cell death has not been significantly affected by the transduction of R9TaRKD-TALE, while showing a higher recovery at 120 h of microspore culture. This work will be helpful to exploit the TALE protein to improve our understanding of DH production and other pathways-related genes. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0254629920311911?via%3Dihub
Počet záznamů: 1