Počet záznamů: 1
Arabidopsis EXO70B2 exocyst subunit contributes to papillae and encasement formation in antifungal defence
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SYSNO ASEP 0557185 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Arabidopsis EXO70B2 exocyst subunit contributes to papillae and encasement formation in antifungal defence Tvůrce(i) Ortmannová, Jitka (UEB-Q) ORCID
Sekereš, Juraj (UEB-Q) RID, ORCID
Kulich, I. (CZ)
Šantrůček, J. (CZ)
Dobrev, Petre (UEB-Q) RID, ORCID
Žárský, Viktor (UEB-Q) RID, ORCID
Pečenková, Tamara (UEB-Q) RID, ORCIDCelkový počet autorů 7 Zdroj.dok. Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 73, č. 3 (2022), s. 742-755Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova nonhost resistance ; callose synthase ; plasma-membrane ; snare-protein ; complex ; transport ; pathogen ; immune ; growth ; identification ; Callose ; exocyst ; non-host resistance ; penetration ; secretion ; snare Obor OECD Cell biology CEP EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000761507000009 EID SCOPUS 85124467058 DOI https://doi.org/10.1093/jxb/erab457 Anotace The exocyst complex containing the EXO70B2 subunit has a specific role in papilla and encasement formation, implemented in coordination with the pathway regulated by the SYP121 SNARE complex in defence. In the reaction to non-adapted Blumeria graminis f. sp. hordei (Bgh), Arabidopsis thaliana leaf epidermal cells deposit cell wall reinforcements called papillae or seal fungal haustoria in encasements, both of which involve intensive exocytosis. A plant syntaxin, SYP121/PEN1, has been found to be of key importance for the timely formation of papillae, and the vesicle tethering complex exocyst subunit EXO70B2 has been found to contribute to their morphology. Here, we identify a specific role for the EXO70B2-containing exocyst complex in the papillae membrane domains important for callose deposition and GFP-SYP121 delivery to the focal attack sites, as well as its contribution to encasement formation. The mRuby2-EXO70B2 co-localizes with the exocyst core subunit SEC6 and GFP-SYP121 in the membrane domain of papillae, and EXO70B2 and SYP121 proteins have the capacity to directly interact. The exo70B2/syp121 double mutant produces a reduced number of papillae and haustorial encasements in response to Bgh, indicating an additive role of the exocyst in SYP121-coordinated non-host resistance. In summary, we report cooperation between the plant exocyst and a SNARE protein in penetration resistance against non-adapted fungal pathogens. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2023 Elektronická adresa http://doi.org/10.1093/jxb/erab457
Počet záznamů: 1