Počet záznamů: 1
Nicotinic Acetylcholine Receptors Expressed by Striatal Interneurons Inhibit Striatal Activity and Control Striatal-Dependent Behaviors
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SYSNO ASEP 0557064 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nicotinic Acetylcholine Receptors Expressed by Striatal Interneurons Inhibit Striatal Activity and Control Striatal-Dependent Behaviors Tvůrce(i) Abbondanza, Alice (FGU-C) ORCID, RID
Ribeiro Bas, Irina (FGU-C) ORCID
Modrák, Martin (MBU-M) ORCID
Čapek, Martin (FGU-C) RID, ORCID
Minich, Jessica (FGU-C)
Tyshkevich, Alexandra (FGU-C)
Naser, Shahed (FGU-C)
Rangotis, Revan (FGU-C) ORCID
Houdek, Pavel (FGU-C) ORCID
Sumová, Alena (FGU-C) RID, ORCID
Dumas, S. (FR)
Bernard, V. (FR)
Janíčková, Helena (FGU-C) RID, ORCID, SAICelkový počet autorů 13 Zdroj.dok. Journal of Neuroscience. - : Society for Neuroscience - ISSN 0270-6474
Roč. 42, č. 13 (2022), s. 2786-2803Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova acetylcholine ; behavior ; Fos ; interneurons ; nAChRs ; striatum Obor OECD Neurosciences (including psychophysiology CEP GJ19-07983Y GA ČR - Grantová agentura ČR LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy 8J21FR022 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018131 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF18_046/0016045 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 ; MBU-M - RVO:61388971 UT WOS 000779618200003 EID SCOPUS 85128160080 DOI https://doi.org/10.1523/JNEUROSCI.1627-21.2022 Anotace Acetylcholine is an important modulator of striatal activity, and it is vital to controlling striatal-dependent behaviors, including motor and cognitive functions. Despite this significance, the mechanisms determining how acetylcholine impacts striatal signaling are still not fully understood. In particular, little is known about the role of nAChRs expressed by striatal interneurons. In the present study, we used FISH to determine which neuronal types express the most prevalent beta2 nicotinic subunit in the mouse striatum. Our data support a common view that nAChR expression is mostly restricted to striatal interneurons. Surprisingly though, cholinergic interneurons were identified as a population with the highest expression of beta2 nicotinic subunit. To investigate the functional significance of beta2containing nAChRs in striatal interneurons, we deleted them by injecting the AAV-Cre vector into the striatum of beta2-flox/flox male mice. The deletion led to alterations in several behavioral domains, namely, to an increased anxiety-like behavior, decrease in sociability ratio, deficit in discrimination learning, and increased amphetamine-induced hyperlocomotion and c-Fos expression in mice with beta2 deletion. Further colocalization analysis showed that the increased c-Fos expression was present in both medium spiny neurons and presumed striatal interneurons. The present study concludes that, despite being relatively rare, beta2-containing nAChRs are primarily expressed in striatal neurons by cholinergic interneurons and play a significant role in behavior. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2023 Elektronická adresa https://doi.org/10.1523/JNEUROSCI.1627-21.2022
Počet záznamů: 1