Počet záznamů: 1
Organosulfur/selenium-based Highly Fluorogenic Molecular Probes for Live-Cell Nucleolus Imaging
- 1.
SYSNO ASEP 0556556 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Organosulfur/selenium-based Highly Fluorogenic Molecular Probes for Live-Cell Nucleolus Imaging Tvůrce(i) Mondal, I. C. (IN)
Galkin, Maksym (UOCHB-X) ORCID
Sharma, S. (IN)
Murugan, N. A. (SE)
Yushchenko, Dmytro A. (UOCHB-X) ORCID, RID
Girdhar, K. (IN)
Karmakar, A. (PT)
Mondal, P. (IN)
Gaur, Pankaj (UOCHB-X) ORCID
Ghosh, S. (IN)Číslo článku e202101281 Zdroj.dok. Chemistry-An Asian Journal . - : Wiley - ISSN 1861-4728
Roč. 17, č. 7 (2022)Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova conformational dynamics ; fluorescent probe ; nucleic acid ; nucleolus imaging ; organochalcogens Obor OECD Biochemistry and molecular biology Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000769575900001 EID SCOPUS 85125380183 DOI 10.1002/asia.202101281 Anotace We present fluorogenic cationic organo chalcogens that are highly selective to RNA. We have demonstrated that the conformational dynamics and subsequently the optical properties of these dyes can be controlled to facilitate efficient bioimaging. We report the application of organoselenium and organosulfur-based cell-permeable red-emissive probes bearing a favorable cyclic sidearm for selective and high contrast imaging of cell nucleoli. The probes exhibit high quantum yield upon interacting with RNA in an aqueous solution. An in-depth multiscale simulation study reveals that the prominent rotational freezing of the electron-donating sidearm of the probes in the microenvironment of RNA helps in attaining more planar conformation when compared to DNA. It exerts a greater extent of intramolecular charge transfer and hence leads to enhanced fluorescence emission. A systematic structure-interaction relationship study highlighted the impact of heavy-chalcogens toward the improved emissive properties of the probes. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2023 Elektronická adresa https://doi.org/10.1002/asia.202101281
Počet záznamů: 1