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Depletion of cardiolipin induces major changes in energy metabolism in Trypanosoma brucei bloodstream forms
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SYSNO ASEP 0554949 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Depletion of cardiolipin induces major changes in energy metabolism in Trypanosoma brucei bloodstream forms Tvůrce(i) Serricchio, M. (CH)
Hierro Yap, Carolina (BC-A)
Schädeli, D. (CH)
Ben Hamidane, H. (QA)
Hemphill, A. (CH)
Graumann, J. (DE)
Zíková, Alena (BC-A) RID, ORCID
Butikofer, P. (CH)Celkový počet autorů 8 Zdroj.dok. FASEB Journal. - : Wiley - ISSN 0892-6638
Roč. 35, č. 2 (2021)Poč.str. 16 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova adp/atp carrier protein ; escherichia-coli ; atp synthase ; heart-mitochondria ; barth-syndrome ; cytochrome-c ; cell-culture ; complex ; growth ; expression ; ATP synthase ; cardiolipin ; electron transport chain ; mitochondria ; protein complexes ; trypanosomes Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA18-17529S GA ČR - Grantová agentura ČR EF16_019/0000759 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000590888700001 EID SCOPUS 85096656101 DOI 10.1096/fj.202001579RR Anotace The mitochondrial inner membrane glycerophospholipid cardiolipin (CL) associates with mitochondrial proteins to regulate their activities and facilitate protein complex and supercomplex formation. Loss of CL leads to destabilized respiratory complexes and mitochondrial dysfunction. The role of CL in an organism lacking a conventional electron transport chain (ETC) has not been elucidated. Trypanosoma brucei bloodstream forms use an unconventional ETC composed of glycerol-3-phosphate dehydrogenase and alternative oxidase (AOX), while the mitochondrial membrane potential (Delta psi m) is generated by the hydrolytic action of the FoF1-ATP synthase (aka FoF1-ATPase). We now report that the inducible depletion of cardiolipin synthase (TbCls) is essential for survival of T brucei bloodstream forms. Loss of CL caused a rapid drop in ATP levels and a decline in the Delta psi m. Unbiased proteomic analyses revealed a reduction in the levels of many mitochondrial proteins, most notably of FoF1-ATPase subunits and AOX, resulting in a strong decline of glycerol-3-phosphate-stimulated oxygen consumption. The changes in cellular respiration preceded the observed decrease in FoF1-ATPase stability, suggesting that the AOX-mediated ETC is the first pathway responding to the decline in CL. Select proteins and pathways involved in glucose and amino acid metabolism were upregulated to counteract the CL depletion-induced drop in cellular ATP. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202001579RR
Počet záznamů: 1