Počet záznamů: 1
Mitochondrial Medicine
- 1.
SYSNO ASEP 0554565 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry Tvůrce(i) Kohutiar, M. (CZ)
Eckhardt, Adam (FGU-C) RID, ORCIDZdroj.dok. Mitochondrial Medicine, Assessing Mitochondria, 2. - New York : Springer, 2021 / Weissig Volkmar ; Edeas Marvin - ISSN 1064-3745 - ISBN 978-1-0716-1266-8 Rozsah stran s. 383-396 Poč.str. 14 s. Poč.str.knihy 459 Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova mitochondria ; nitrotyrosine ; 2D electrophoresis ; immunoblotting ; mass spectrometry Obor OECD Biochemical research methods CEP NV17-31564A GA MZd - Ministerstvo zdravotnictví Institucionální podpora FGU-C - RVO:67985823 UT WOS 000893349700029 EID SCOPUS 85107188199 DOI https://doi.org/10.1007/978-1-0716-1266-8_28 Anotace Nitrotyrosine formation is caused by presence of reactive oxygen and nitrogen species. Nitration is a very selective process leading to specific modification of only a few tyrosines in protein molecule. 2D electrophoresis and western blotting techniques coupled with mass spectrometry are common methods used in analysis of proteome. Here we describe protocol for analysis of peroxynitrite-induced protein nitration in isolated mitochondria. Mitochondrial proteins are separated by 2D electrophoresis and transferred to nitrocellulose membrane. Membranes are then incubated with antibodies against nitrotyrosine. Positive spots are compared with corresponding Coomassie-stained gels, and protein nitration is confirmed with mass spectrometry techniques. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://link.springer.com/protocol/10.1007/978-1-0716-1266-8_28
Počet záznamů: 1