Počet záznamů: 1
Polychlorinated environmental toxicants affect sphingolipid metabolism during neurogenesis in vitro
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SYSNO ASEP 0554413 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Polychlorinated environmental toxicants affect sphingolipid metabolism during neurogenesis in vitro Tvůrce(i) Slováčková, J. (CZ)
Slavík, J. (CZ)
Kulich, P. (CZ)
Večeřa, J. (CZ)
Kováč, O. (CZ)
Paculova, H. (CZ)
Straková, N. (CZ)
Fedr, Radek (BFU-R) ORCID
Silva, J. P. (PT)
Carvalho, F. (PT)
Machala, M. (CZ)
Procházková, Jiřina (BFU-R) RID, ORCIDCelkový počet autorů 12 Číslo článku 152986 Zdroj.dok. Toxicology. - : Elsevier - ISSN 0300-483X
Roč. 463, NOV 2021 (2021)Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. IE - Irsko Klíč. slova junctional intercellular communication ; ceramide 1-phosphate ; pcb congeners ; biphenyls ; sulfatide ; cells Vědní obor RIV FR - Farmakologie a lékárnická chemie Obor OECD Pharmacology and pharmacy CEP GA21-11585S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BFU-R - RVO:68081707 UT WOS 000709560800013 DOI 10.1016/j.tox.2021.152986 Anotace Sphingolipids (SLs) are important signaling molecules and functional components of cellular membranes. Although SLs are known as crucial regulators of neural cell physiology and differentiation, modulations of SLs by environmental neurotoxicants in neural cells and their neuronal progeny have not yet been explored. In this study, we used in vitro models of differentiated neuron-like cells, which were repeatedly exposed during differentiation to model environmental toxicants, and we analyzed changes in sphingolipidome, cellular morphology and gene expression related to SL metabolism or neuronal differentiation. We compared these data with the results obtained in undifferentiated neural cells with progenitor-like features. As model polychlorinated organic pollutants, we used 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3,3'-dichlorobiphenyl (PCB11) and 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153). PCB153 revealed itself as the most prominent deregulator of SL metabolism and as potent toxicant during early phases of in vitro neurogenesis. TCDD exerted only minor changes in the levels of analysed lipid species, however, it significantly changed the rate of pro-neuronal differentiation and deregulated expression of neuronal markers during neurogenesis. PCB11 acted as a potent disruptor of in vitro neurogenesis, which induced significant alterations in SL metabolism and cellular morphology in both differentiated neuron-like models (differentiated NE4C and NG108-15 cells). We identified ceramide-1-phosphate, lactosylceramides and several glycosphingolipids to be the most sensitive SL species to exposure to polychlorinated pollutants. Additionally, we identified deregulation of several genes related to SL metabolism, which may be explored in future as potential markers of developmental neurotoxicity. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0300483X21003085?via%3Dihub
Počet záznamů: 1