Počet záznamů: 1
Comparative Study Highlights the Potential of Spectral Deconvolution for Fucoxanthin Screening in Live Phaeodactylum tricornutum Cultures
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SYSNO ASEP 0553567 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Comparative Study Highlights the Potential of Spectral Deconvolution for Fucoxanthin Screening in Live Phaeodactylum tricornutum Cultures Tvůrce(i) Macdonald Miller, S. (AU)
Abbriano, R. (AU)
Segečová, Anna (UEK-B) SAI, RID, ORCID
Herdean, A. (AU)
Ralph, P. J. (AU)
Pernice, M. (AU)Celkový počet autorů 6 Číslo článku 19 Zdroj.dok. Marine Drugs. - : MDPI
Roč. 20, č. 1 (2022)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova chlorophylls ; quantification ; fucoxanthin ; microalgae ; green consumption ; food consumption Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora UEK-B - RVO:86652079 UT WOS 000750547900001 EID SCOPUS 85121651108 DOI 10.3390/md20010019 Anotace Microalgal biotechnology shows considerable promise as a sustainable contributor to a broad range of industrial avenues. The field is however limited by processing methods that have commonly hindered the progress of high throughput screening, and consequently development of improved microalgal strains. We tested various microplate reader and flow cytometer methods for monitoring the commercially relevant pigment fucoxanthin in the marine diatom Phaeodactylum tricornutum. Based on accuracy and flexibility, we chose one described previously to adapt to live culture samples using a microplate reader and achieved a high correlation to HPLC (R-2 = 0.849), effectively removing the need for solvent extraction. This was achieved by using new absorbance spectra inputs, reducing the detectable pigment library and changing pathlength values for the spectral deconvolution method in microplate reader format. Adaptation to 384-well microplates and removal of the need to equalize cultures by density further increased the screening rate. This work is of primary interest to projects requiring detection of biological pigments, and could theoretically be extended to other organisms and pigments of interest, improving the viability of microalgae biotechnology as a contributor to sustainable industry. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/1660-3397/20/1/19
Počet záznamů: 1