Počet záznamů: 1
Rapid Determination of Metronidazole and 2-Hydroxymetronidazole in Murine Blood Plasma
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SYSNO ASEP 0553498 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Rapid Determination of Metronidazole and 2-Hydroxymetronidazole in Murine Blood Plasma Tvůrce(i) Zemanová, N. (CZ)
Anzenbacher, P. (CZ)
Hudcovic, Tomáš (MBU-M) RID, ORCID
Anzenbacherová, E. (CZ)Zdroj.dok. Journal of Chromatographic Science. - : Oxford University Press - ISSN 0021-9665
Roč. 60, č. 1 (2022), s. 81-87Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova performance liquid-chromatography ; saliva ; assay ; validation Vědní obor RIV EC - Imunologie Obor OECD Immunology CEP GA19-08294S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000747657200010 EID SCOPUS 85118874194 DOI 10.1093/chromsci/bmab049 Anotace Metronidazole is a drug used to treat bacterial and protozoan infections. Nowadays, it is one of the most frequently prescribed drugs worldwide. The main aim of this paper is to present a rapid, reliable and simple high-performance liquid chromatography (HPLC) method to determine metronidazole along with its primary metabolite, 2-hydroxymetronidazole, in plasma or serum using paracetamol as an internal standard. A total of 100% methanol was used to denature plasma proteins. After centrifugation, the supernatant was evaporated under nitrogen flow. The samples were dissolved in the mobile phase and injected into a Li-Chrospher RP-18 column. A total of 10 mmol/L NaH2PO4: acetonitrile (90:10, v/v) solution with a flow rate of 1 mL/min was used as the mobile phase. Metronidazole and 2-hydroxymetronidazole were detected at two different wavelengths at 320 nm and 311 nm, respectively. The method is characterized by high precision (relative standard deviation % < 6). The method was used for the determination of metronidazole and 2-hydroxymetronidazole in murine blood using small amounts of plasma (<= 100 mu L). Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://academic.oup.com/chromsci/article-abstract/60/1/81/6238591?redirectedFrom=fulltext&login=true
Počet záznamů: 1