Počet záznamů: 1  

Rapid Determination of Metronidazole and 2-Hydroxymetronidazole in Murine Blood Plasma

  1. 1.
    SYSNO ASEP0553498
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevRapid Determination of Metronidazole and 2-Hydroxymetronidazole in Murine Blood Plasma
    Tvůrce(i) Zemanová, N. (CZ)
    Anzenbacher, P. (CZ)
    Hudcovic, Tomáš (MBU-M) RID, ORCID
    Anzenbacherová, E. (CZ)
    Zdroj.dok.Journal of Chromatographic Science. - : Oxford University Press - ISSN 0021-9665
    Roč. 60, č. 1 (2022), s. 81-87
    Poč.str.7 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaperformance liquid-chromatography ; saliva ; assay ; validation
    Vědní obor RIVEC - Imunologie
    Obor OECDImmunology
    CEPGA19-08294S GA ČR - Grantová agentura ČR
    Způsob publikováníOpen access
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000747657200010
    EID SCOPUS85118874194
    DOI10.1093/chromsci/bmab049
    AnotaceMetronidazole is a drug used to treat bacterial and protozoan infections. Nowadays, it is one of the most frequently prescribed drugs worldwide. The main aim of this paper is to present a rapid, reliable and simple high-performance liquid chromatography (HPLC) method to determine metronidazole along with its primary metabolite, 2-hydroxymetronidazole, in plasma or serum using paracetamol as an internal standard. A total of 100% methanol was used to denature plasma proteins. After centrifugation, the supernatant was evaporated under nitrogen flow. The samples were dissolved in the mobile phase and injected into a Li-Chrospher RP-18 column. A total of 10 mmol/L NaH2PO4: acetonitrile (90:10, v/v) solution with a flow rate of 1 mL/min was used as the mobile phase. Metronidazole and 2-hydroxymetronidazole were detected at two different wavelengths at 320 nm and 311 nm, respectively. The method is characterized by high precision (relative standard deviation % < 6). The method was used for the determination of metronidazole and 2-hydroxymetronidazole in murine blood using small amounts of plasma (<= 100 mu L).
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2023
    Elektronická adresahttps://academic.oup.com/chromsci/article-abstract/60/1/81/6238591?redirectedFrom=fulltext&login=true
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.