Počet záznamů: 1
Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana
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SYSNO ASEP 0552833 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana Tvůrce(i) Kashkan, Ivan (UEB-Q) ORCID
Hrtyan, M. (CZ)
Retzer, Katarzyna (UEB-Q) ORCID
Humpolíčková, Jana (UOCHB-X) ORCID
Jayasree, A. (CZ)
Filepová, Roberta (UEB-Q) SAI
Vondráková, Zuzana (UEB-Q) RID, ORCID
Simon, Sibu (UEB-Q) ORCID
Rombaut, D. (BE)
Jacobs, T. B. (BE)
Frilander, M. J. (AT)
Hejátko, J. (CZ)
Friml, J. (AT)
Petrášek, Jan (UEB-Q) RID, ORCID
Růžička, Kamil (UEB-Q) ORCIDCelkový počet autorů 15 Zdroj.dok. New Phytologist - ISSN 0028-646X
Roč. 233, č. 1 (2022), s. 329-343Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova alternative splicing ; Arabidopsis thaliana ; auxin ; auxin transport ; frap ; PINs ; plant development ; RNA processing Obor OECD Developmental biology CEP GA19-23773S GA ČR - Grantová agentura ČR GA19-18917S GA ČR - Grantová agentura ČR EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_013/0001775 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 ; UOCHB-X - RVO:61388963 UT WOS 000714678100001 EID SCOPUS 85118492063 DOI 10.1111/nph.17792 Anotace Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.1111/nph.17792
Počet záznamů: 1