Počet záznamů: 1
Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.)
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SYSNO ASEP 0552702 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.) Tvůrce(i) Gatarira, C. (NG)
Sládeková, Lucia (UEB-Q)
Němečková, Alžběta (UEB-Q) ORCID
Šimoníková, Denisa (UEB-Q) ORCID
Paliwal, R. (NG)
Asfaw, A. (NG)
Abberton, M. (NG)
Badara, G. (NG)
Asiedu, R. (NG)
Čížková, Jana (UEB-Q) RID, ORCID
Hřibová, Eva (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
DeKoeyer, D. (CA)
Adetimirin, V. (NG)
Bhattacharjee, R. (NG)Celkový počet autorů 15 Číslo článku 1897 Zdroj.dok. Agronomy. - : MDPI - ISSN 2073-4395
Roč. 11, č. 10 (2021)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova nuclear-DNA content ; flow-cytometry ; genetic diversity ; alata l. ; plants ; microsatellite ; musa ; relatedness ; association ; polyploidy ; flow cytometry ; chromosome counting ; ploidy level ; DArTseq Obor OECD Biochemistry and molecular biology CEP EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000711881200001 EID SCOPUS 85126816369 DOI https://doi.org/10.3390/agronomy11101897 Anotace Yam (Dioscorea spp.) is a monocotyledonous herbaceous vine plant grown in the tropics and subtropics. It is a multi-species plant with varied intra- and interspecific ploidy levels. Of the 600 species, 11 are cultivated supporting the livelihood of over 300 million people. The paucity of information on ploidy and the genomic constitution is a significant challenge to the crop's genetic improvement through crossbreeding. The objective of this study was to investigate the ploidy levels of 236 accessions across six cultivated and two wild species using chromosome counting, flow cytometry and genotyping-based ploidy determination methods. Results obtained from chromosome counting and genotyping-based ploidy determination were in agreement. In majority of the accessions, chromosome counting and flow cytometry were congruent, allowing future rapid screening of ploidy levels using flow cytometry. Among cultivated accessions, 168 (71%) were diploid, 50 (21%) were triploid, and 12 (5%) were tetraploid. Two wild species included in the study were diploids. Resolution of ploidy level in yams offers opportunities for implementing successful breeding programmes through intra- and interspecific hybridization. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.3390/agronomy11101897
Počet záznamů: 1