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Antioxidative Effect of Dietary Flavonoid Isoquercitrin on Human Ovarian Granulosa Cells HGL5 In Vitro
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SYSNO ASEP 0550825 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Antioxidative Effect of Dietary Flavonoid Isoquercitrin on Human Ovarian Granulosa Cells HGL5 In Vitro Tvůrce(i) Kolesárová, A. (SK)
Michalcová, K. (SK)
Roychoudhury, S. (IN)
Baldovská, S. (SK)
Tvrdá, E. (SK)
Vašíček, J. (SK)
Chrenek, P. (SK)
Sanislo, L. (SK)
Křen, Vladimír (MBU-M) RID, ORCIDZdroj.dok. Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 70, č. 5 (2021), s. 745-754Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova Flavonoid ; Granulosa cells ; Antioxidant ; Steroid hormones ; Transforming growth factor Vědní obor RIV FR - Farmakologie a lékárnická chemie Obor OECD Pharmacology and pharmacy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000729810300008 EID SCOPUS 85122310132 DOI 10.33549/physiolres.934692 Anotace This study aimed to examine the effect of dietary flavonoid isoquercitrin on ovarian granulosa cells using the immortalized human cell line HGL5. Cell viability, survival, apoptosis, release of steroid hormones 17 beta-estradiol and progesterone, and human transforming growth factor-beta 2 (TGF-beta 2) and TGF-beta 2 receptor as well as intracellular reactive oxygen species (ROS) generation were investigated after isoquercitrin treatment at the concentration range of 5-100 mu g.ml(-1). It did not cause any significant change (p>0.05) in cell viability as studied by AlamarBlue assay in comparison to control. No significant change was observed (p>0.05) in the proportion of live, dead and apoptotic cells as revealed by apoptotic assay using flow cytometry. Similarly, the release of 17 beta-estradiol, progesterone, TGF-beta 2 and its receptor were not affected significantly (p>0.05) by isoquercitrin as detected by ELISA, in comparison to control. Except for the highest concentration of 100 mu g.ml(-1), which led to oxidative stress, isoquercitrin exhibited antioxidative activity at lower concentration used in the study (5, 10, 25, and 50 mu g.ml(-1)) by hampering the production of intracellular ROS, in comparison to control, as detected by chemiluminescence assay (p<0.05). Findings of the present study indicate an existence of the antioxidative pathway that involves inhibition of intracellular ROS generation by isoquercitrin in human ovarian granulosa cells. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.biomed.cas.cz/physiolres/pdf/2021/70_745.pdf
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