Počet záznamů: 1  

Antioxidative Effect of Dietary Flavonoid Isoquercitrin on Human Ovarian Granulosa Cells HGL5 In Vitro

  1. 1.
    SYSNO ASEP0550825
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevAntioxidative Effect of Dietary Flavonoid Isoquercitrin on Human Ovarian Granulosa Cells HGL5 In Vitro
    Tvůrce(i) Kolesárová, A. (SK)
    Michalcová, K. (SK)
    Roychoudhury, S. (IN)
    Baldovská, S. (SK)
    Tvrdá, E. (SK)
    Vašíček, J. (SK)
    Chrenek, P. (SK)
    Sanislo, L. (SK)
    Křen, Vladimír (MBU-M) RID, ORCID
    Zdroj.dok.Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
    Roč. 70, č. 5 (2021), s. 745-754
    Poč.str.10 s.
    Jazyk dok.eng - angličtina
    Země vyd.CZ - Česká republika
    Klíč. slovaFlavonoid ; Granulosa cells ; Antioxidant ; Steroid hormones ; Transforming growth factor
    Vědní obor RIVFR - Farmakologie a lékárnická chemie
    Obor OECDPharmacology and pharmacy
    Způsob publikováníOpen access
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000729810300008
    EID SCOPUS85122310132
    DOI10.33549/physiolres.934692
    AnotaceThis study aimed to examine the effect of dietary flavonoid isoquercitrin on ovarian granulosa cells using the immortalized human cell line HGL5. Cell viability, survival, apoptosis, release of steroid hormones 17 beta-estradiol and progesterone, and human transforming growth factor-beta 2 (TGF-beta 2) and TGF-beta 2 receptor as well as intracellular reactive oxygen species (ROS) generation were investigated after isoquercitrin treatment at the concentration range of 5-100 mu g.ml(-1). It did not cause any significant change (p>0.05) in cell viability as studied by AlamarBlue assay in comparison to control. No significant change was observed (p>0.05) in the proportion of live, dead and apoptotic cells as revealed by apoptotic assay using flow cytometry. Similarly, the release of 17 beta-estradiol, progesterone, TGF-beta 2 and its receptor were not affected significantly (p>0.05) by isoquercitrin as detected by ELISA, in comparison to control. Except for the highest concentration of 100 mu g.ml(-1), which led to oxidative stress, isoquercitrin exhibited antioxidative activity at lower concentration used in the study (5, 10, 25, and 50 mu g.ml(-1)) by hampering the production of intracellular ROS, in comparison to control, as detected by chemiluminescence assay (p<0.05). Findings of the present study indicate an existence of the antioxidative pathway that involves inhibition of intracellular ROS generation by isoquercitrin in human ovarian granulosa cells.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2022
    Elektronická adresahttps://www.biomed.cas.cz/physiolres/pdf/2021/70_745.pdf
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.