Počet záznamů: 1
Secretory IgAN-glycans contribute to the protection againstE. coliO55 infection of germ-free piglets
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SYSNO ASEP 0550089 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Secretory IgAN-glycans contribute to the protection againstE. coliO55 infection of germ-free piglets Tvůrce(i) Raskova Kafkova, L. (CZ)
Brokešová, D. (CZ)
Křupka, M. (CZ)
Stehlíková, Zuzana (MBU-M)
Dvořák, Jiří (MBU-M) RID, ORCID
Coufal, Štěpán (MBU-M) ORCID, RID
Fajstová, Alena (MBU-M) ORCID
Šrůtková, Dagmar (MBU-M) ORCID, RID
Štěpánová, Kateřina (MBU-M) ORCID
Hermanová, Petra (MBU-M) ORCID
Štěpánková, Renata (MBU-M) RID
Überall, I. (CZ)
Škarda, J. (CZ)
Novák, Z. (US)
Vannucci, Luca (MBU-M) RID, ORCID
Tlaskalová-Hogenová, Helena (MBU-M) RID, ORCID
Jirásková Zákostelská, Zuzana (MBU-M) ORCID
Šinkora, Marek (MBU-M) RID, ORCID
Městecký, Jiří (MBU-M) ORCID
Raška, M. (CZ)Zdroj.dok. Mucosal Immunology. - : Springer - ISSN 1933-0219
Roč. 14, č. 2 (2021), s. 511-522Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova escherichia-coli ; immunoglobulin-a ; o-glycosylation ; small-intestine ; hinge-region ; n-glycan ; component ; antibodies ; binding ; pig Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA17-11275S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000572598900001 EID SCOPUS 85091687047 DOI https://doi.org/10.1038/s41385-020-00345-8 Anotace Mucosal surfaces are colonized by highly diverse commensal microbiota. Coating with secretory IgA (SIgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens. Bacterial coating could be mediated by antigen-specific SIgA recognition, polyreactivity, and/or by the SIgA-associated glycans. In contrast to many in vitro studies, only a few reported the effect of SIgA glycans in vivo. Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removedN-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenicE. coliO55 challenge. SIgA, Fab, and Fc-SC were protective, whereas removal ofN-glycans from SIgA reduced SIgA-mediated protection as demonstrated by piglets' intestinal histology, clinical status, and survival. In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination ofE. coliO55. These findings highlight the role of SIgA-associatedN-glycans in protection. Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.nature.com/articles/s41385-020-00345-8
Počet záznamů: 1