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Actin Cytoskeleton Regulation by the Yeast NADPH Oxidase Yno1p Impacts Processes Controlled by MAPK Pathways
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SYSNO ASEP 0549644 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Actin Cytoskeleton Regulation by the Yeast NADPH Oxidase Yno1p Impacts Processes Controlled by MAPK Pathways Tvůrce(i) Weber, M. (AT)
Basu, S. (US)
Gonzalez, B. (US)
Greslehner, G. P. (AT)
Singer, S. (AT)
Hašková, Danuša (MBU-M)
Hašek, Jiří (MBU-M) RID, ORCID
Breitenbach, M. (AT)
Gourlay, C. W. (GB)
Cullen, P. J. (US)
Rinnerthaler, M. (AT)Číslo článku 322 Zdroj.dok. Antioxidants. - : MDPI
Roč. 10, č. 2 (2021)Poč.str. 25 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova ros ; MAP kinase pathway ; actin ; osmotic stress ; filamentous growth ; invasive growth ; pseudohyphal growth ; pheromone response ; apoptosis Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000622060700001 EID SCOPUS 85101227073 DOI https://doi.org/10.3390/antiox10020322 Anotace Reactive oxygen species (ROS) that exceed the antioxidative capacity of the cell can be harmful and are termed oxidative stress. Increasing evidence suggests that ROS are not exclusively detrimental, but can fulfill important signaling functions. Recently, we have been able to demonstrate that a NADPH oxidase-like enzyme (termed Yno1p) exists in the single-celled organism Saccharomyces cerevisiae. This enzyme resides in the peripheral and perinuclear endoplasmic reticulum and functions in close proximity to the plasma membrane. Its product, hydrogen peroxide, which is also produced by the action of the superoxide dismutase, Sod1p, influences signaling of key regulatory proteins Ras2p and Yck1p/2p. In the present work, we demonstrate that Yno1p-derived H2O2 regulates outputs controlled by three MAP kinase pathways that can share components: the filamentous growth (filamentous growth MAPK (fMAPK)), pheromone response, and osmotic stress response (hyperosmolarity glycerol response, HOG) pathways. A key structural component and regulator in this process is the actin cytoskeleton. The nucleation and stabilization of actin are regulated by Yno1p. Cells lacking YNO1 showed reduced invasive growth, which could be reversed by stimulation of actin nucleation. Additionally, under osmotic stress, the vacuoles of a increment yno1 strain show an enhanced fragmentation. During pheromone response induced by the addition of alpha-factor, Yno1p is responsible for a burst of ROS. Collectively, these results broaden the roles of ROS to encompass microbial differentiation responses and stress responses controlled by MAPK pathways. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2076-3921/10/2/322
Počet záznamů: 1