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Effects of heat treatment on metabolism of tobacco plants infected with Potato virus Y
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SYSNO ASEP 0549630 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effects of heat treatment on metabolism of tobacco plants infected with Potato virus Y Tvůrce(i) Hýsková, V. (CZ)
Bělonožníková, K. (CZ)
Doričová, V. (CZ)
Kavan, D. (CZ)
Gillarová, S. (CZ)
Henke, S. (DE)
Synková, Helena (UEB-Q) RID, ORCID
Ryšlavá, H. (CZ)
Čeřovská, Noemi (UEB-Q) RID, ORCIDCelkový počet autorů 9 Zdroj.dok. Plant Biology. - : Wiley - ISSN 1435-8603
Roč. 23, č. 1 (2021), s. 131-141Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova glycosidases ; Hatch–Slack cycle ; heat shock ; Hsp70 ; Hsp90 ; phenolic acids ; Potato virus Y Obor OECD Biochemical research methods CEP EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000614432100001 EID SCOPUS 85100328799 DOI https://doi.org/10.1111/plb.13234 Anotace Many factors affect successful virus propagation and plant defence responses. Heat shock protein (Hsp) expression after heat shock plays an ambiguous role in viral infection. On the one hand, Hsp70 participates in plant defence response, on the other hand, Hsp70 could interact with viral proteins and facilitate virus propagation. Here, we studied metabolic adaptations of Nicotiana tabacum L. subjected to heat shock (42 °C, 2 h) before or after inoculating the plants with Potato virus Y (potyvirus). RT-qPCR and ELISA were used for potyvirus quantification. Hsp70 and Hsp90 isoforms were analysed by Western blotting. Salicylic, quinic and chlorogenic acid content was determined by LC-MS. The activity of Hatch–Slack enzymes (as markers of potyviral infection in tobacco) and glycosidases was assayed. Application of heat shock before or after inoculation showed accelerated potyviral propagation in comparison with only inoculated plants. Plants exposed to heat shock and concurrently inoculated showed higher potyviral content, higher amount of Hsp70, together with late decline of quinic acid content and low chlorogenic acid content. Spread of potyviral infection correlated with enhanced salicylic acid content and activities of enzymes of the Hatch–Slack cycle, α- and β-galactosidase, α-mannosidase, α-glucosidase and β-N-acetylhexosaminidase. Heat shock proteins accelerate potyviral propagation. The lower weight cytosolic and mitochondrial Hsp70 (~50-75 kDa) persist throughout the viral infection. Also, the plant defense response results in increase of salicylic and chlorogenic acids but decrease of quinic acid content. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.1111/plb.13234
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