0549157 - ÚEB 2022 RIV CH eng J - Článek v odborném periodiku
Raspor, M. - Motyka, Václav - Kaleri, A. R. - Ninković, S. - Tubić, L. - Cingel, A. - Ćosić, T.
Integrating the roles for cytokinin and auxin in de novo shoot organogenesis: From hormone uptake to signaling outputs.
International Journal of Molecular Sciences. Roč. 22, č. 16 (2021), č. článku 8554. E-ISSN 1422-0067
Grant CEP: GA ČR GA19-13103S
Institucionální podpora: RVO:61389030
Klíčová slova: Auxin * Cytokinin * De novo shoot organogenesis * dnso * Gene regulatory network * Hormone uptake * Shoot regeneration * Sucrose * Transport
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 6.208, rok: 2021
Způsob publikování: Open access
http://doi.org/10.3390/ijms22168554

De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this process, and genes involved in their signaling cascades act as master regulators of the different phases of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration are often interpreted as a consequence of these hormones simply being present in the regeneration media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon source, plays a signaling role and has been recently shown to interact with CK and auxin and to affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the regeneration media and their interaction with sucrose present in the media to their complex signaling outputs that mediate shoot regeneration.
Trvalý link: http://hdl.handle.net/11104/0325168