Počet záznamů: 1
Noninvasive Combined Diagnosis and Monitoring of Aspergillus and Pseudomonas Infections: Proof of Concept
- 1.
SYSNO ASEP 0547360 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Noninvasive Combined Diagnosis and Monitoring of Aspergillus and Pseudomonas Infections: Proof of Concept Tvůrce(i) Dobiáš, R. (CZ)
Škríba, Anton (MBU-M) ORCID
Pluháček, Tomáš (MBU-M) ORCID
Petřík, M. (CZ)
Palyzová, Andrea (MBU-M) RID, ORCID
Káňová, M. (CZ)
Čubova, E. (CZ)
Houšť, Jiří (MBU-M) ORCID
Novák, Jiří (MBU-M) RID, ORCID
Stevens, D. A. (US)
Mitulovic, G. (AT)
Krejčí, E. (CZ)
Hubáček, P. (CZ)
Havlíček, Vladimír (MBU-M) RID, ORCIDČíslo článku 730 Zdroj.dok. Journal of Fungi. - : MDPI
Roč. 7, č. 9 (2021)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Aspergillus fumigatus ; Pseudomonas aeruginosa ; invasive infection ; noninvasive diagnosis ; coinfection ; virulence factor ; siderophores ; quorum-sensing molecules Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA19-10907S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000699735300001 EID SCOPUS 85114829758 DOI 10.3390/jof7090730 Anotace In acutely ill patients, particularly in intensive care units or in mixed infections, time to a microbe-specific diagnosis is critical to a successful outcome of therapy. We report the application of evolving technologies involving mass spectrometry to diagnose and monitor a patient's course. As proof of this concept, we studied five patients and used two rat models of mono-infection and coinfection. We report the noninvasive combined monitoring of Aspergillus fumigatus and Pseudomonas aeruginosa infection. The invasive coinfection was detected by monitoring the fungal triacetylfusarinine C and ferricrocin siderophore levels and the bacterial metabolites pyoverdin E, pyochelin, and 2-heptyl-4-quinolone, studied in the urine, endotracheal aspirate, or breath condensate. The coinfection was monitored by mass spectrometry followed by isotopic data filtering. In the rat infection model, detection indicated 100-fold more siderophores in urine compared to sera, indicating the diagnostic potential of urine sampling. The tools utilized in our studies can now be examined in large clinical series, where we could expect the accuracy and speed of diagnosis to be competitive with conventional methods and provide advantages in unraveling the complexities of mixed infections. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2309-608X/7/9/730
Počet záznamů: 1