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Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements.
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SYSNO ASEP 0547305 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements. Tvůrce(i) Zajíc, J. (CZ)
Ripp, S. (US)
Trögl, J. (CZ)
Kuncová, Gabriela (UCHP-M) RID, SAI
Pospíšilová, M. (CZ)Zdroj.dok. International Conference on Sensors and Nanotechnology IEEE /1./. - New York : Institute of Electrical and Electronics Engineers Inc., 2019 - ISBN 978-1-5386-5619-8 Rozsah stran s. 185-188 Poč.str. 4 s. Forma vydání Online - E Akce IEEE International Conference on Sensors and Nanotechnology Datum konání 24.07.2019 - 25.07.2019 Místo konání Penang Země MY - Malajsie Typ akce WRD Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova biosensor ; bioluminescent bioreporter ; optical fiber sensor Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management Institucionální podpora UCHP-M - RVO:67985858 UT WOS 000525734000047 EID SCOPUS 85078161809 DOI 10.1109/SENSORSNANO44414.2019.8940080 Anotace A whole-cell optical fiber sensor of toluene was prepared by the adsorption of Pseudomonas putida TVA8, the bacterial strain producing light in contact with toluene, in the form of biofilm. Bioreporter immobilization was facilitated by (3-Aminopropyl)triethoxysilane (APTES) treatment on the wider end of tapered quartz optical fiber element (OFE). Repeatability of preparation and response of the active part of the biosensor was demonstrated in five trials with different OFE geometries in which the bioluminescence (BL) was induced with toluene solution (26.5 mg L-1) for 2 weeks. Longevity of inductions of the biosensor was shown in over 135 days trial, when the biosensor was repetitively induced with toluene solution (26.5 mg L-1) 68 times. The intensities of BL gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of BL induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene. Ideal shape of OFE, which maximizes the detected signal, was calculated to be a Frustrum cone. The developed sensor is plausible for multiple and fast online monitoring of the presence of a pollutant in remote localities but not its concentration. Pracoviště Ústav chemických procesů Kontakt Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Rok sběru 2022 Elektronická adresa https://ieeexplore.ieee.org/document/8940080
Počet záznamů: 1