Počet záznamů: 1
A novel liquid chromatography/mass spectrometry method for determination of neurotransmitters in brain tissue: Application to human tauopathies
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SYSNO ASEP 0545437 Druh ASEP J - Článek v odborném periodiku Zařazení RIV Záznam nebyl označen do RIV Poddruh J Článek ve WOS Název A novel liquid chromatography/mass spectrometry method for determination of neurotransmitters in brain tissue: Application to human tauopathies Tvůrce(i) Forgáčová, A.
Galba, J. (SK)
Garruto, R.M. (US)
Majerová, P. (SK)
Katina, Stanislav (UIVT-O) SAI, ORCID, RID
Kováč, A.Celkový počet autorů 6 Zdroj.dok. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232
Roč. 1073 (2018), s. 154-162Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova tandem mass-spectrometry ; amino-acid neurotransmitters ; alzheimers-disease brain ; cerebrospinal-fluid ; rat-brain ; monoamine neurotransmitters ; precolumn derivatization ; metabolites ; samples ; plasma ; Neurotransmitters ; uhplc/ms ; Human brain tissue ; Tauopathies UT WOS 000423889300020 EID SCOPUS 85038847196 DOI 10.1016/j.jchromb.2017.12.015 Anotace Neurotransmitters, small molecules widely distributed in the central nervous system are essential in transmitting electrical signals across neurons via chemical communication. Dysregulation of these chemical signaling molecules is linked to numerous neurological diseases including tauopathies. In this study, a precise and reliable liquid chromatography method was established with tandem mass spectrometry detection for the simultaneous determination of aspartic acid, asparagine, glutamic acid, glutamine, gamma-aminobutyric acid, N-acetyl-L-aspartic acid, pyroglutamic acid, acetylcholine and choline in human brain tissue. The method was successfully applied to the analysis of human brain tissues from three different tauopathies; corticobasal degeneration, progressive supranuclear palsy and parkinsonism-dementia complex of Guam. Neurotransmitters were analyzed on ultra-high performance chromatography (UHPLC) using an ethylene bridged hybrid amide column coupled with tandem mass spectrometry (MS/MS). Identification and quantification of neurotransmitters was carried out by ESI+ mass spectrometry detection. We optimized sample preparation to achieve simple and fast extraction of all nine analytes. Our method exhibited an excellent linearity for all analytes (all coefficients of determination > 0.99), with inter-day and intra-day precision yielding relative standard deviations 3.2%-41.2% and an accuracy was in range of 92.6%-104.3%. The present study, using the above method, is the first to demonstrate significant alterations of brain neurotransmitters caused by pathological processes in the brain tissues of patient with three different tauopathies. Pracoviště Ústav informatiky Kontakt Tereza Šírová, sirova@cs.cas.cz, Tel.: 266 053 800 Rok sběru 2022
Počet záznamů: 1