Počet záznamů: 1
Human osteoblast-like SAOS-2 cells on submicron-scale fibers coated with nanocrystalline diamond films
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SYSNO ASEP 0541653 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Human osteoblast-like SAOS-2 cells on submicron-scale fibers coated with nanocrystalline diamond films Tvůrce(i) Steinerová, Marie (FGU-C) ORCID, RID
Matějka, Roman (FGU-C) RID, ORCID, SAI
Štěpanovská, Jana (FGU-C) ORCID, RID
Filová, Elena (FGU-C) RID, ORCID
Staňková, Ľubica (FGU-C) RID, ORCID
Rysová, M. (CZ)
Martinová, L. (CZ)
Dragounová, Helena (FGU-C)
Domonkos, Mária (FZU-D) RID
Artemenko, Anna (FZU-D) RID, ORCID
Babchenko, Oleg (FZU-D) RID, ORCID
Otáhal, M. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCID
Kromka, Alexander (FZU-D) RID, ORCID, SAIČíslo článku 111792 Zdroj.dok. Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931
Roč. 121, Feb (2021)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova nanocrystalline diamond ; submicron fibers ; hierarchical organization ; silica nanostructures ; osteoblast proliferation ; osteogenic differentiation ; collagen ; alkaline phosphatase ; osteocalcin Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Biomaterials (as related to medical implants, devices, sensors) Vědní obor RIV – spolupráce Fyzikální ústav - Biofyzika CEP GA19-02891S GA ČR - Grantová agentura ČR NV19-02-00068 GA MZd - Ministerstvo zdravotnictví EF16_019/0000760 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018110 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CzechNanoLab - 90110 - Vysoké učení technické v Brně Způsob publikování Omezený přístup Institucionální podpora FGU-C - RVO:67985823 ; FZU-D - RVO:68378271 UT WOS 000619124600007 EID SCOPUS 85098711683 DOI https://doi.org/10.1016/j.msec.2020.111792 Anotace A unique composite nanodiamond-based porous material with a hierarchically-organized submicron-nanostructure was constructed for potential bone tissue engineering. This material consisted of submicron fibers prepared by electrospinning of silicon oxide (SiOx), which were oxygen-terminated (O-SiOx) and were hermetically coated with nanocrystalline diamond (NCD) films. The NCD films were then terminated with hydrogen (HNCD) or oxygen (O-NCD). The materials were tested as substrates for the adhesion, growth and osteogenic differentiation of human osteoblast-like Saos-2 cells. The number and the spreading area of the initially adhered cells, their growth rate during 7 days after seeding and the activity of alkaline phosphatase (ALP) were significantly higher on the NCD-coated samples than on the uncoated 0-SiO x samples. In addition, the concentration of type I collagen was significantly higher in the cells on the O-NCD-coated samples than on the bare O-SiOx samples. The observed differences could be attributed to the tunable wettability of NCD and to the more appropriate surface morphology of the NCD-coated samples in contrast to the less stable, rapidly eroding bare SiOx surface. The H-NCD coatings and the O-NCD coatings both promoted similar initial adhesion of Saos-2 cells, but the subsequent cell proliferation activity was higher on the O-NCD-coated samples. The concentration of beta-actin, vinculin, type I collagen and alkaline phosphatase (ALP), the ALP activity, and also the calcium deposition tended to be higher in the cells on the O-NCD-coated samples than on the H-NCD-coated samples, although these differences did not reach statistical significance. The improved cell performance on the O-NCD-coated samples could be attributed to higher wettability of these samples (water drop contact angle less than 10 degrees), while the H-NCD-coated samples were hydrophobic (contact angle >701. NCD-coated porous SiOx meshes can therefore be considered as appropriate scaffolds for bone tissue engineering, particularly those with an O-terminated NCD coating. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://doi.org/10.1016/j.msec.2020.111792
Počet záznamů: 1