Počet záznamů: 1
Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars
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SYSNO ASEP 0541299 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars Tvůrce(i) Lakhneko, O. (SK)
Danchenko, M. (SK)
Morgun, B. (UA)
Kovac, A. (SK)
Majerova, P. (SK)
Škultéty, L'udovít (MBU-M) ORCID, RIDČíslo článku 3445 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI - ISSN 1661-6596
Roč. 21, č. 10 (2020)Poč.str. 21 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova amylase-trypsin-inhibitors ; triticum-aestivum l. ; proteomic analysis ; celiac-disease ; Triticum aestivum L ; food quality ; cereal allergens ; discovery proteomics ; gluten ; celiac disease Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000539312100041 EID SCOPUS 85084786514 DOI https://doi.org/10.3390/ijms21103445 Anotace Bread wheat (Triticum aestivum L.) is one of the most valuable cereal crops for human consumption. Its grain storage proteins define bread quality, though they may cause food intolerances or allergies in susceptible individuals. Herein, we discovered a diversity of grain proteins in three Ukrainian wheat cultivars: Sotnytsia, Panna (both modern selection), and Ukrainka (landrace). Firstly, proteins were isolated with a detergent-containing buffer that allowed extraction of various groups of storage proteins (glutenins, gliadins, globulins, and albumins), secondly, the proteome was profiled by the two-dimensional gel electrophoresis. Using multi-enzymatic digestion, we identified 49 differentially accumulated proteins. Parallel ultrahigh-performance liquid chromatography separation followed by direct mass spectrometry quantification complemented the results. Principal component analysis confirmed that differences among genotypes were a major source of variation. Non-gluten fraction better discriminated bread wheat cultivars. Various accumulation of clinically relevant plant proteins highlighted one of the modern genotypes as a promising donor for the breeding of hypoallergenic cereals. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/1422-0067/21/10/3445
Počet záznamů: 1