Počet záznamů: 1
The Rho guanine nucleotide exchange factor Trio is required for neural crest cell migration and interacts with Dishevelled
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SYSNO ASEP 0540503 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Rho guanine nucleotide exchange factor Trio is required for neural crest cell migration and interacts with Dishevelled Tvůrce(i) Kratzer, M.-C. (DE)
Becker, S. F. S. (FR)
Grund, A. (DE)
Merks, A. (DE)
Harnoš, J. (CZ)
Bryja, Vítězslav (BFU-R) RID, ORCID
Giehl, K. (DE)
Kashef, J. (DE)
Borchers, A. (DE)Celkový počet autorů 9 Číslo článku dev186338 Zdroj.dok. Development. - : Company of Biologists - ISSN 0950-1991
Roč. 147, č. 10 (2020)Poč.str. 15 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova intellectual disability ; signaling pathways ; beta-catenin ; gef domain ; xenopus ; rac Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000541757600011 EID SCOPUS 85085535229 DOI 10.1242/dev.186338 Anotace Directional migration during embryogenesis and tumor progression faces the challenge that numerous external signals need to converge to precisely control cell movement. The Rho guanine exchange factor (GEF) Trio is especially well suited to relay signals, as it features distinct catalytic domains to activate Rho GTPases. Here, we show that Trio is required for Xenopus cranial neural crest (NC) cell migration and cartilage formation. Trio cell-autonomously controls protrusion formation of NC cells and Trio morphant NC cells show a blebbing phenotype. Interestingly, the Trio GEF2 domain is sufficient to rescue protrusion formation and migration of Trio morphant NC cells. We show that this domain interacts with the DEP/C-terminus of Dishevelled (DVL). DVL but not a deletion construct lacking the DEP domain is able to rescue protrusion formation and migration of Trio morphant NC cells. This is likely mediated by activation of Reel, as we find that DVL rescues Rac1 activity in Trio morphant embryos. Thus, our data provide evidence for a novel signaling pathway, whereby Trio controls protrusion formation of cranial NC cells by interacting with DVL to activate Rac1. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2021 Elektronická adresa https://dev.biologists.org/content/147/10/dev186338
Počet záznamů: 1