Počet záznamů: 1
Karyotype changes in long-term cultured tick cell lines
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SYSNO ASEP 0538574 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Karyotype changes in long-term cultured tick cell lines Tvůrce(i) Kotsarenko, Kateryna (BC-A) ORCID
Věchtová, Pavlína (BC-A) RID, ORCID
Lieskovská, J. (CZ)
Füssy, Zoltán (BC-A) RID, ORCID
Cabral-de-Mello, D.C. (BR)
Rego, Ryan O. M. (BC-A) RID, ORCID
Alberdi, P. (ES)
Collinson, M. (GB)
Bell-Sakyi, L. (GB)
Štěrba, Ján (BC-A) RID, ORCID
Grubhoffer, Libor (BC-A) RID, ORCIDCelkový počet autorů 11 Číslo článku 13443 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 10, č. 1 (2020)Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova transgene expression ; ixodes-scapularis ; genome size ; acari ; evolution ; insect ; establishment ; adaptation ; discovery ; repeats Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP LTARF18021 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA15-03044S GA ČR - Grantová agentura ČR GA18-27204S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000561123700019 EID SCOPUS 85089275095 DOI 10.1038/s41598-020-70330-5 Anotace Tick cell lines are an easy-to-handle system for the study of viral and bacterial infections and other aspects of tick cellular processes. Tick cell cultures are often continuously cultivated, as freezing can affect their viability. However, the long-term cultivation of tick cells can influence their genome stability. In the present study, we investigated karyotype and genome size of tick cell lines. Though 16S rDNA sequencing showed the similarity between Ixodes spp. cell lines at different passages, their karyotypes differed from 2n=28 chromosomes for parental Ixodes spp. ticks, and both increase and decrease in chromosome numbers were observed. For example, the highly passaged Ixodes scapularis cell line ISE18 and Ixodes ricinus cell lines IRE/CTVM19 and IRE/CTVM20 had modal chromosome numbers 48, 23 and 48, respectively. Also, the Ornithodoros moubata cell line OME/CTVM22 had the modal chromosome number 33 instead of 2n=20 chromosomes for Ornithodoros spp. ticks. All studied tick cell lines had a larger genome size in comparison to the genomes of the parental ticks. Thus, highly passaged tick cell lines can be used for research purposes, but possible differences in encoded genetic information and downstream cellular processes, between different cell populations, should be taken into account. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2021 Elektronická adresa https://www.nature.com/articles/s41598-020-70330-5
Počet záznamů: 1